2020年7月
Functional annotation of human long noncoding RNAs via molecular phenotyping.
Genome research
- 巻
- 30
- 号
- 7
- 開始ページ
- 1060
- 終了ページ
- 1072
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1101/gr.254219.119
Long noncoding RNAs (lncRNAs) constitute the majority of transcripts in the mammalian genomes, and yet, their functions remain largely unknown. As part of the FANTOM6 project, we systematically knocked down the expression of 285 lncRNAs in human dermal fibroblasts and quantified cellular growth, morphological changes, and transcriptomic responses using Capped Analysis of Gene Expression (CAGE). Antisense oligonucleotides targeting the same lncRNAs exhibited global concordance, and the molecular phenotype, measured by CAGE, recapitulated the observed cellular phenotypes while providing additional insights on the affected genes and pathways. Here, we disseminate the largest-to-date lncRNA knockdown data set with molecular phenotyping (over 1000 CAGE deep-sequencing libraries) for further exploration and highlight functional roles for ZNF213-AS1 and lnc-KHDC3L-2.
- リンク情報
- ID情報
-
- DOI : 10.1101/gr.254219.119
- ORCIDのPut Code : 60189856
- PubMed ID : 32718982
- PubMed Central 記事ID : PMC7397864