Applied Entomology and Zoology 4(1) 101-107 Feb 2019 [Refereed]
The identification of insect species is generally achieved by analyzing the specific morphological characteristics or, in more recent years, by polymerase chain reaction (PCR) assays of DNA sequences. PCR assays, which detect specific segments of ...
Collection of Amphibolus venator (Klug) by a trap baited with brown rice in a rice milling factory on Okinawa-jima Island, Okinawa, Japan
Taro IMAMURA, Akihiro MIYANOSHITA, Mayumi NIREGI and Satoshi FURUI
Urban Pest Management 8(1) 11-14 Jun 2018 [Refereed]
農研機構研究報告 食品研究部門 = Bulletin of the NARO,Food Research (2) 27-34 Mar 2018
The number of stored-product insects captured by traps at the outdoors was examined from November 2016 to October 2017 in Tsukuba. The total numbers of Plodia interpunctella (Hilbner) (Lepidoptera; Pyralidae), Lasioderma serricorne (Fabricius) (Co...
農研機構研究報告 食品研究部門 = Bulletin of the NARO, Food Research (1) 65-71 Mar 2017
つくば市の屋外で捕獲される貯穀害虫の発生状況と消長を明らかにするため，2015年の11月から翌2016年の10月にかけてトラップ調査を引き続き行った．その結果，ノシメマダラメイガ，タバコシバンムシ，ヒメマルカツオブシムシは４個のトラップで，それぞれ総計75個体，59個体，72個体が捕獲された．コクヌストモドキは過去２年間捕獲されなかったが，2016年度は総計15個体が捕獲された．The number of stored-product insects captured by traps a...
<p>The Indian meal moth, Plodia interpunctella (Hübner), frequently contaminates cup-packaged instant noodle products. Such products consist of dried food materials, noodles, vegetables, meats, and soup powder. The larvae living in the cup ...
<p>We collected and counted overwintering maize weevils, Sitophilus zeamais, in outdoor brown-rice warehouses in Ibaraki Prefecture, Japan. The survey was conducted at 5 sites on 10 December 2015. We found S. zeamais adults under pie...
<p>We investigated the larval development of the Indian meal moth Plodia interpunctella (Lepidoptera: Pyralidae) (Hübner) at 28°C, 70％ RH, and 16L 8D on a Japanese rice cracker (kaki-no-tane), fried peanuts, and brown rice. Kaki-n...
We investigated the larval development of the Indian meal moth Plodia interpunctella (Lepidoptera: Pyralidae) (Hubner) at 28℃, 70% RH, and 16 L 8D on dried foods for cats and birds (parakeets). Firstinstar larvae could not develop on intact dried ...
We investigated the larval development of the Indian meal moth, Plodia interpunctella (Lepidoptera: Pyralidae) (Hubner), on roasted whole almonds covered with milk chocolate, white chocolate, or milk chocolate containing broken kaki-no-tane at 28℃...
The number and distribution of Plodia interpunctella (Hubner) (Lepidoptera; Pyralidae) captured by sex pheromone traps at or near a condominium were examined from mid August to mid September 2011 in Tsukuba, Ibaraki Prefecture. The average daily n...
To study impacts of various random effects and parameters of collaborative studies on the precision of quantitation methods of genetically modified (GM) crops, we developed a set of random effects models for cycle time values of a standard curveba...
Qualitative PCR methods for the genetically modified (GM) maize events MON810, Bt11, and GA21, and the 35S promoter (P35S) region of the cauliflower mosaic virus (CaMV) were evaluated in an interlaboratory study. Real-time PCR-based quantitative m...
Biological & Pharmaceutical Bulletin 36(1) 131-134 Jan 2013
A novel real-time polymerase chain reaction (PCR)-based quantitative screening method was developed for three genetically modified soybeans: RRS, A2704-12, and MON89788. The 35S promoter (P35S) of cauliflower mosaic virus is introduced into RRS an...
To develop a method for detecting GM wheat that may be marketed in the near future, we evaluated the proline-rich protein (PRP) gene as an endogenous reference gene of common wheat (Triticum aestivum L.) and durum wheat (Tr...
MATSUOKA Yasuyuki, KITTA Kazumi, ARAMI Shin-ichiro, SATO Megumi, HARAGUCHI Hiroyuki, KURIMOTO Youichi, IMAI Shinjiro, TANAKA Keiko, MANO Junichi, FURUI Satoshi
J. Food Hyg. Soc. Jpn 53(5) 195-202 Oct 2012
A PCR-based method was developed to distinguish between durum/common wheat and common wheat by leveraging slight differences of DNA sequence in Starch Synthase II (SS II) coded on wheat A, B and D genomes. A primer pair, SS II ex7-U/L, was ...
We have developed a real-time PCR array method to comprehensively detect genetically modified (GM) organisms. In the method, genomic DNA extracted from an agricultural product is analyzed using various qualitative real-time PCR assays on a 96-well...
A GM maize event, MIR604, has been widely distributed and an analytical method to quantify its content is required to monitor the validity of food labeling. Here we report a novel real-time PCR-based quantitation method for MIR604 maize. We develo...
Journal of Agricultural and Food Chemistry 59 6856-6863 Jul 2011
Because of the increasing use of maize hybrids with genetically modified (GM) stacked events, the established and commonly used bulk sample methods for PCR quantification of GM maize in non-GM maize are prone to overestimate the GM organism (GMO) ...
A novel real-time PCR-based analytical method was developed for the event-specific quantification of a genetically modified (GM) soybean event; A2704-12. During the plant transformation, DNA fragments derived from pUC19 plasmid were integrated in ...
Quantitative and qualitative methods based on PCR have been developed for genetically modified organisms (GMO). Interlaboratory studies were previously conducted for GMO quantitative methods; in this study, an interlaboratory study was conducted f...
To reduce the cost and time required to routinely perform the genetically modified organism (GMO) test, we developed a duplex quantitative real-time PCR method for a screening analysis simultaneously targeting an event-specific segment for GA21 an...
A novel real-time PCR-based analytical method was established for the event-specific quantification of a GM soybean event MON89788. The conversion factor (Cf) which is required to calculate the GMO amount was experimentally deter...
Food Science and Technology Research 16 421-430 Sep 2010
Novel real-time PCR-based quantitative methods were developed for three GM maize events; MON863, NK603 and TC1507. The quantitative methods were designed to amplify an event-specific segment for MON863 and NK603, and a construct-specific segment f...
A novel multiplex PCR method was developed for simultaneous event-specific detection of four events of GM maize, i.e., DAS-59122-7, MIR604, MON88017, and MON863. The single laboratory examination of analytical performance using simulated DN...
A real-time polymerase chain reaction (PCR) method specific for genetically modified (GM) maize event DAS59132 (E32) was adapted for qualitative detection of low level presence of E32. The method was validated by a collaborative trial with eight p...
Analytical and Bioanalytical Chemistry 396 457-464 Jan 2010
DNA analysis with the polymerase chain reaction (PCR) has become a routine part of medical diagnostics, environmental inspections, food evaluations, and biological studies. Furthermore, the development of a microscale PCR chip is an essential comp...
Polypropylene microtubes (tubes) are generally used for bio-material tests in addition to PCR tests such as genetically modified organism (GMO) testings. However, the choice of suitable tubes is quite important, because it might influence the resu...
The Bt11 maize-specific qualitative detection method based on polymerase chain reaction (PCR) in the JAS analytical test handbook has been widely used for administrative monitoring of GM crops and quality control of commercially distributed grains...
Biological & Pharmaceutical Bulletin 32(11) 1824-1829 Nov 2009
To screen for unauthorized genetically modified organisms (GMO) in the various crops, we developed a multiplex real-time polymerase chain reaction high-resolution melting-curve analysis method for the simultaneous qualitative detection of 35S prom...
A duplex real-time PCR method was developed for quantitative screening analysis of GM maize. The duplex real-time PCR simultaneously detected two GM-specific segments, namely the cauliflower mosaic virus (CaMV) 35S promoter (P35S) segment and an e...
In this study, we are reporting for the first time an efficient, accurate and inexpensive rapid detection system which employs the integration of isothermal amplification and subsequent analysis of unpurified amplicons by an electrochemical system...
Journal of Agricultural and Food Chemistry 57 2640-2646 Apr 2009
We developed a multiplex polymerase chain reaction (PCR)-multiplex ligase chain reaction (LCR) (MPCR-MLCR) technique as a novel approach for the simultaneous detection of recombinant DNA segments (e.g., promoters, trait genes, and terminators) of ...
Genetically modified (GM) sugar beets have been bred for use as food and animal feed. To evaluate the applicability of GMO analyses to beet sugar products, we investigated residual DNA in eight sorts of in-process beet sugar samples and commercial...
Journal of Agricultural and Food Chemistry 57 26-37 Jan 2009
We developed a novel type of real-time polymerase chain reaction (PCR) array with TaqMan chemistry as a platform for the comprehensive and semiquantitative detection of genetically modified (GM) crops. Thirty primer-probe sets for the specific det...
Real-time polymerase chain reaction (PCR)-based quantitative methods were previously developed and validated for genetically modified (GM) maize or soy. In this study, the quantification step of the validated methods was modified, and an interlabo...
Detection methods using a conventional polymerase chain reaction (PCR) were developed to detect two lines of genetically modified (GM) rice that have not been approved as safe for use in foods in Japan. Two GM rice lines were Bacillus thuringiensi...
Journal of Agricultural and Food Chemistry 56 5521-5527 Jul 2008
A novel type of quantitative competitive polymerase chain reaction (QC-PCR) system for the detection and quantification of the Roundup Ready soybean (RRS) was developed. This system was designed based on the advantage of a fully validated real-tim...
Journal of Agricultural and Food Chemistry 56 1977-1983 Mar 2008
In many countries, the labeling of grains and feed- and foodstuffs is mandatory if the genetically modified organism (GMO) content exceeds a certain level of approved GM varieties. The GMO content in a maize sample containing the combined-trait (s...
A real-time PCR detection method was developed for event-specific quantitation of Roundup Ready® maize, GA21. The developed PCR method was designed to amplify an artificial junction site between the native maize genome DNA and the r...
We developed a specific method to extract DNA from rice grain samples and modified the qualitative real-time PCR method provided by Bayer Co., Ltd. for reliable detection of the genetically modified (GM) rice variety, LLRice601, which has not unde...
Journal of Agricultural and Food Chemistry 55 5942-5947 Jul 2007
We analyzed the DNA fragments extracted from four rice vermicelli products. The Bacillus thuringiensis (Bt) rice line, which has a construct similar to the GM Shanyou 63 line, was detected in some vermicelli products by identification of the junct...
Philippe Corbisier, Wim Broothaerts, Sabrina Gioria, Heinz Schimmel, Malcolm Burns, Anna Baoutina, Kerry R. Emslie, Satoshi Furui, Yasunori Kurosawa, Marcia J. Holden, Hyong Ha Kim, Yun Mi Lee, Mamoru Kawaharasaki, Della Sin, Jing Wang
Journal of Agricultural and Food Chemistry 55 3249-3257 May 2007
An international CCQM-P60 pilot study involving eight national metrological institutes was organized to investigate if the quantification of genetically modified (GM) corn powder by real-time PCR was affected by the DNA extraction method applied. ...
Journal of Agricultural and Food Chemistry 55 1274-1279 Feb 2007
In 2005 it was reported that the genetically modified (GM) maize strain or 'event' called Bt10 had been distributed inadvertently in the United States over the previous 4 years. In order to ensure that grain for food and feed production did not co...
Analytical and Bioanalytical Chemistry 386 1327-1333 Nov 2006
Polymerase chain reaction (PCR) is an essential part of research based on genomics or cell analysis. The development of a microfluidic device that would be suitable for high-temperature-based reactions therefore becomes an important contribution t...
Journal of Agricultural and Food Chemistry 53 9713-9721 Dec 2005
In this study, we developed a novel multiplex polymerase chain reaction (PCR) method for simultaneous detection of up to eight events of genetically modified (GM) maize within a single reaction. The eight detection primer pairs designed to be cons...
Journal of Agricultural and Food Chemistry 53 6294-6300 Aug 2005
Qualitative and quantitative Polymerase Chain Reaction (PCR) systems aimed at the specific detection and quantification of common wheat DNA are described. Many countries have issued regulations to label foods that include genetically modified orga...
Journal of Agricultural and Food Chemistry 53 2052-2059 Mar 2005
The applicability of quantifying genetically modified (GM) maize and soy to processed foods was investigated using heat treatment processing models. The detection methods were based on real-time quantitative polymerase chain reaction (PCR) analysi...
It is very important to avoid contaminations when we perform tests to detect genetically modified organisms (GMO) using methods based on PCR techniques. Heat-treatment by autoclaving is usually recommended in the testing protocol to sterilize reag...
Bioscience, biotechnology, and biochemistry 67(5) 1160-1163 May 2003
Transglucosylation activities of spinach α-glucosidase I and IV, which have different substrate specificity for hydrolyzing activity, were investigated. In a maltose mixture, α-glucosidase I, which has high activity toward not only maltooligosacch...
Bioscience, biotechnology, and biochemistry 61(8) 1379-1381 Aug 1997
A cDNA encoding spinach putative phospholipid hydroperoxide glutathione peroxidase (PHGPX) was cloned and sequenced. The cDNA included an open reading frame that encoded a polypeptide of 171 amino acid residues. The deduced amino acid sequence sho...
Bioscience, biotechnology, and biochemistry 61(8) 1382-1383 Aug 1997
The rate parameters for maltooligosaccharides of two α-glucosidases from spinach seeds were examined and subsite affinities were evaluated. The subsite affinities for subsites 1, 2, 3, 4, 5, 6, and 7 in the active site of α-glucosidase A and B wer...
Bioscience, biotechnology, and biochemistry 59(4) 673-677 Apr 1995
Four molecular forms of α-glucosidase were isolated from spinach seeds by several kinds of chromatography. The molecular masses of α-glucosidases I, II, III, and IV were 78, 78, 82, and 82kDa by SDS-PAGE, and 62, 62, 190, and 70kDa by gel filtrati...
Two molecular forms of α-glucosidase were isolated from spinach seeds after storage at 4℃ by CM-cellulose column chromatography and gel filtration. The molecular masses of α-glucosidase A and B were 78 kDa and 82 kDa by SDS-PAGE, and 62 kDa and 70...
Four molecular forms of α-glucosidase were isolated from spinach seeds by several kinds of chromatography. The molecular masses of α-glucosidases Ⅰ,Ⅱ,Ⅲ,and Ⅳ were 78,78,82 and 82kDa by SDS-PAGE, and 62,62,190,and 70kDa by gel filtration, respectiv...
