1994年2月
CHARACTERIZATION OF A RANA-CATESBEIANA LECTIN-RESISTANT MUTANT OF LEUKEMIA P388 CELLS
CANCER RESEARCH
- 巻
- 54
- 号
- 4
- 開始ページ
- 928
- 終了ページ
- 934
- 記述言語
- 英語
- 掲載種別
- 出版者・発行元
- AMER ASSOC CANCER RESEARCH
Sialic acid-binding lectin (SBL-C) from Rana catesbeiana eggs inhibits the growth of tumor cells such as P388 and L1210 leukemia cells (K. Nitta et at, Cancer Res., 54: 920-927, 1994). Here we report the establishment of an SBL-resistant P388 variant cell line, RC-150. Both P388 and RC-150 cells were agglutinated by SBL-C; however, growth of RC-150 cells was unaffected by SBL-C. Cytoplasmic free Ca2+ concentration and transglutaminase activity of RC-150 cells were 0.5 (110 nM) and 3 times (0.62 nmol/mg/min) as high as those of P388 cells, respectively. Microvilli and microplicae were observed on the surface of P388 cells by scanning electron microscopy but were rarely seen on RC-150 cells. Dansylcadaverine-labeled SBL-C bound to both P388 and RC-150 cells. Binding of SBL-C to these tumor cells appears to be mediated by two species of wheat germ agglutinin-stained cell membrane sialoglycoproteins. Labeled SBL-C entered P388 but not RC-150 cells, suggesting that internalized SBL-C acts as an inhibitor of cell proliferation.
- リンク情報
- ID情報
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- ISSN : 0008-5472
- Web of Science ID : WOS:A1994MW68600016