2014年10月
Sucutinirane-diterpene derivatives induce apoptosis via oxidative stress in HL-60 cells
JOURNAL OF NATURAL MEDICINES
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- 巻
- 68
- 号
- 4
- 開始ページ
- 723
- 終了ページ
- 729
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/s11418-014-0848-6
- 出版者・発行元
- SPRINGER JAPAN KK
Previously, we reported the isolation of cassane-type diterpenes, sucutiniranes A-F, from the seeds of Bowdichia nitida. In this study, a series of sucutinirane derivatives was prepared, and their in vitro toxicity in the HL-60 cell line was evaluated. Then the action mechanism of a representative compound that induces cell death was investigated. Whereas C-6 or C-7 diol esters and ether decreased the activity against the HL-60 cell line, furan-oxidized derivatives 12 and 13 showed improvement or retention of the activity compared with those of the natural products sucutinirane A (11), E (1), and F (2). Treatment with sucutinirane derivative 13 elevated caspase 3/7 activity and also decreased expression of Bcl-2 family proteins, Mcl-1, and Bid. Derivative 13 generated reactive oxygen species in HL-60 cells, whose apoptotic effects were attenuated by the addition of an antioxidant, N-acetyl-l-cysteine. These results suggest that cassane butenolide 13 induces apoptosis in HL-60 via its oxidative effects.
- リンク情報
- ID情報
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- DOI : 10.1007/s11418-014-0848-6
- ISSN : 1340-3443
- eISSN : 1861-0293
- Web of Science ID : WOS:000341879000008