論文

査読有り
2016年8月

Oocyte-dependent activation of MTOR in cumulus cells controls the development and survival of cumulus-oocyte complexes

JOURNAL OF CELL SCIENCE
  • Jing Guo
  • ,
  • Lanying Shi
  • ,
  • Xuhong Gong
  • ,
  • Mengjie Jiang
  • ,
  • Yaoxue Yin
  • ,
  • Xiaoyun Zhang
  • ,
  • Hong Yin
  • ,
  • Hui Li
  • ,
  • Chihiro Emori
  • ,
  • Koji Sugiura
  • ,
  • John J. Eppig
  • ,
  • You-Qiang Su

129
16
開始ページ
3091
終了ページ
3103
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1242/jcs.182642
出版者・発行元
COMPANY OF BIOLOGISTS LTD

Communication between oocytes and their companion somatic cells promotes the healthy development of ovarian follicles, which is crucial for producing oocytes that can be fertilized and are competent to support embryogenesis. However, how oocyte-derived signaling regulates these essential processes remains largely undefined. Here, we demonstrate that oocyte-derived paracrine factors, particularly GDF9 and GDF9- BMP15 heterodimer, promote the development and survival of cumulus-cell-oocyte complexes (COCs), partly by suppressing the expression of Ddit4l, a negative regulator of MTOR, and enabling the activation of MTOR signaling in cumulus cells. Cumulus cells expressed less Ddit4l mRNA and protein than mural granulosa cells, which is in striking contrast to the expression of phosphorylated RPS6 (a major downstream effector of MTOR). Knockdown of Ddit4l activated MTOR signaling in cumulus cells, whereas inhibition of MTOR in COCs compromised oocyte developmental competence and cumulus cell survival, with the latter likely to be attributable to specific changes in a subset of transcripts in the transcriptome of COCs. Therefore, oocyte suppression of Ddit4l expression allows for MTOR activation in cumulus cells, and this oocyte-dependent activation of MTOR signaling in cumulus cells controls the development and survival of COCs.

Web of Science ® 被引用回数 : 18

リンク情報
DOI
https://doi.org/10.1242/jcs.182642
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27358481
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000384079900006&DestApp=WOS_CPL