論文

査読有り
2016年8月

Mouse oocytes suppress miR-322-5p expression in ovarian granulosa cells

JOURNAL OF REPRODUCTION AND DEVELOPMENT
  • Jun-ichi Sumitomo
  • ,
  • Chihiro Emori
  • ,
  • Yuta Matsuno
  • ,
  • Mizuki Ueno
  • ,
  • Kurenai Kawasaki
  • ,
  • Takaho A. Endo
  • ,
  • Katsuyuki Shiroguchi
  • ,
  • Wataru Fujii
  • ,
  • Kunihiko Naito
  • ,
  • Koji Sugiura

62
4
開始ページ
393
終了ページ
399
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1262/jrd.2015-161
出版者・発行元
SOCIETY REPRODUCTION & DEVELOPMENT-SRD

This study tested the hypothesis that oocyte-derived paracrine factors (ODPFs) regulate miRNA expression in mouse granulosa cells. Expression of mmu-miR-322-5p (miR-322) was higher in mural granulosa cells (MGCs) than in cumulus cells of the Graafian follicles. The expression levels of miR-322 decreased when cumulus cells or MGCs were co-cultured with oocytes denuded of their cumulus cells. Inhibition of SMAD2/3 signaling by SB431542 increased miR-322 expression by cumulus-oocyte complexes (COCs). Moreover, the cumulus cells but not the MGCs in Bmp15(-/-)Gdf9(+/-)(double-mutant) mice exhibited higher miR-322 expression than those of wild-type mice. Taken together, these results show that ODPFs suppress the expression of miR-322 in cumulus cells. Gene ontology analysis of putative miR-322 targets whose expression was detected in MGCs with RNA-sequencing suggested that multiple biological processes are affected by miR-322 in MGCs. These results demonstrate that ODPFs regulate miRNA expression in granulosa cells and that this regulation may participate in the differential control of cumulus cell versus MGC functions. Therefore, the ODPF-mediated regulation of cumulus cells takes place at both transcriptional and post-transcriptional levels.

Web of Science ® 被引用回数 : 3

リンク情報
DOI
https://doi.org/10.1262/jrd.2015-161
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27180925
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000382598700012&DestApp=WOS_CPL