論文

査読有り
2016年9月

Effects of porcine oocytes on the expression levels of transcripts encoding glycolytic enzymes in granulosa cells

ANIMAL SCIENCE JOURNAL
  • Yuta Matsuno
  • ,
  • Asuka Onuma
  • ,
  • Yoshie A. Fujioka
  • ,
  • Chihiro Emori
  • ,
  • Wataru Fujii
  • ,
  • Kunihiko Naito
  • ,
  • Koji Sugiura

87
9
開始ページ
1114
終了ページ
1121
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/asj.12539
出版者・発行元
WILEY

Oocytes play critical roles in regulating the expression of transcripts encoding the glycolytic enzymes phosphofructokinase, platelet (PFKP) and lactate dehydrogenase A (LDHA) in granulosa cells in mice, but whether this is the case in pigs or other mammals has not been adequately investigated. Therefore, the aim of this study was to determine whether porcine oocytes regulate the expression levels of these transcripts in granulosa cells in vitro. Porcine cumulus cells expressed higher levels of PFKP and LDHA transcripts than mural granulosa cells (MGCs). However, co-culturing with oocytes had no significant effect on the isolated cumulus cells. While murine oocytes promoted the expression of both Pfkp and Ldha transcripts by murine MGCs, porcine oocytes promoted the expression of only Pfkp, but not Ldha transcripts by murine MGCs. Neither murine nor porcine oocytes affected PFKP and LDHA expression by porcine MGCs. Moreover, in the presence of porcine follicular fluid, porcine oocytes maintained the expression of PFKP, but not LDHA by porcine cumulus cells. Therefore, porcine oocytes are capable of regulating the expression of PFKP but not LDHA in granulosa cells in coordination with unknown factor(s) present in the follicular fluid.

Web of Science ® 被引用回数 : 2

リンク情報
DOI
https://doi.org/10.1111/asj.12539
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/26560349
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000383630300007&DestApp=WOS_CPL