2018年11月30日
Diffracted X-ray Blinking Tracks Single Protein Motions.
Scientific reports
- 巻
- 8
- 号
- 1
- 開始ページ
- 17090
- 終了ページ
- 17090
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/s41598-018-35468-3
- 出版者・発行元
- NATURE PUBLISHING GROUP
Single molecule dynamics studies have begun to use quantum probes. Single particle analysis using cryo-transmission electron microscopy has dramatically improved the resolution when studying protein structures and is shifting towards molecular motion observations. X-ray free-electron lasers are also being explored as routes for determining single molecule structures of biological entities. Here, we propose a new X-ray single molecule technology that allows observation of molecular internal motion over long time scales, ranging from milliseconds up to 103 seconds. Our method uses both low-dose monochromatic X-rays and nanocrystal labelling technology. During monochromatic X-ray diffraction experiments, the intensity of X-ray diffraction from moving single nanocrystals appears to blink because of Brownian motion in aqueous solutions. X-ray diffraction spots from moving nanocrystals were observed to cycle in and out of the Bragg condition. Consequently, the internal motions of a protein molecule labelled with nanocrystals could be extracted from the time trajectory using this diffracted X-ray blinking (DXB) approach. Finally, we succeeded in distinguishing the degree of fluctuation motions of an individual acetylcholine-binding protein (AChBP) interacting with acetylcholine (ACh) using a laboratory X-ray source.
- リンク情報
-
- DOI
- https://doi.org/10.1038/s41598-018-35468-3
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/30504916
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269541
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000451746700001&DestApp=WOS_CPL
- ID情報
-
- DOI : 10.1038/s41598-018-35468-3
- ISSN : 2045-2322
- PubMed ID : 30504916
- PubMed Central 記事ID : PMC6269541
- Web of Science ID : WOS:000451746700001