Tomoyuki Yasukawa

J-GLOBAL         Last updated: Oct 30, 2018 at 09:38
 
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Name
Tomoyuki Yasukawa
E-mail
yasusci.u-hyogo.ac.jp
Affiliation
University of Hyogo
Section
Graduate Schoolof Material Science

Research Areas

 
 

Academic & Professional Experience

 
Jul 2001
 - 
Jan 2013
PhD, Department of Electronics & Electrical Engineering, University of Glasgow
 
Jul 2000
 - 
Jun 2001
Research Fellow, Department of Electronics & Electrical Engineering, University of Glasgow, The Royal Society of Chemistry (U.K.)
 

Awards & Honors

 
Nov 2007
Negative Dielectrophoretic Manipulation with Microparticles for Rapid Immunosensing, Best Poster Award, 2008 International Symposium on Micro-NanoMechatronics and Human Science (MHS2008)
 
May 1999
Fabrication and Characterization of Dual-Microdisk Electrode for Simultaneous Detection of Two Electroactive Species Around a Single Cell, Best Presentation Award, Asian Conference on Electrochemistry in 1999
 

Published Papers

 
Junya Yoshioka, Yu Ohsugi, Yu Ohsugi, Toru Yoshitomi, Tomoyuki Yasukawa, Naoki Sasaki, Keitaro Yoshimoto, Keitaro Yoshimoto
Sensors (Switzerland)   18(9)    Sep 2018   [Refereed]
© 2018 by the authors. Licensee MDPI, Basel, Switzerland. Bone marrow-derived mesenchymal stem cells (BMSCs) are an important cell resource for stem cell-based therapy, which are generally isolated and enriched by the density-gradient method based...
安川智之
静電気学会誌   42(4) 162‐167   Jul 2018   [Refereed][Invited]
Takatomo Sugano, Yui Sasaki, Fumio Mizutani, Tomoyuki Yasukawa
Analytical Sciences   34(2) 127-130   Jan 2018   [Refereed]
© The Japan Society for Analytical Chemistry. Arrays with cell aggregations and single-cell arrays embedded in hydrogel sheets were fabricated by negative dielectrophoretic (n-DEP) cell-manipulation techniques and hydrogel gelation. Cells suspende...
Rapid enrichment and mechanical stimulation of mesenchymal stem cells using dielectrophoresis devices
Yoshioka Junya, Ohsugi Yu, Yoshitomi Toru, Yasukawa Tomoyuki, Sasaki Naoki, Yoshimoto Keitaro
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY   255    Mar 2018   [Refereed]
安川智之
ぶんせき   (9) 418‐424   Sep 2017   [Refereed][Invited]
Kohei Tominaga, Satoshi Arimoto, Ken Shimono, Toshihiko Yoshioka, Fumio Mizutani, Tomoyuki Yasukawa
Analytical Sciences   33(4) 531-536   Jan 2017   [Refereed]
© The Japan Society for Analytical Chemistry. A single-step electrochemical immunochromatography has been developed: the device was based on two pieces of nitrocellulose membrane, a sample pad with anti-mouse IgG antibody labeled with glucose oxid...
Toshiki Hokuto, Tomoyuki Yasukawa, Ryota Kunikata, Atsushi Suda, Kumi Y. Inoue, Kosuke Ino, Tomokazu Matsue, Tomokazu Matsue, Fumio Mizutani
Biotechnology Journal   11(6) 838-842   Jun 2016   [Refereed]
Copyright © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Electrochemical imaging is an excellent technique to characterize an activity of biomaterials, such as enzymes and cells. Large scale integration-based amperometric sensor (Bio-LSI) has...
Taishu Tanaka, Fumio Mizutani, Tomoyuki Yasukawa
Electrochemistry   84(5) 361-363   May 2016   [Refereed]
© 2016 The Electrochemical Society of Japan, All rights reserved. We describe here the dielectrophoretic manipulation using a microdisk electrode with microcavity for picking up, positioning and relocating single target cells. The attractive force...
Junya Yoshioka, Toru Yoshitomi, Tomoyuki Yasukawa, Keitaro Yoshimoto
Analytical Sciences   32(11) 1213-1216   Jan 2016   [Refereed]
© Japan Society for Analytical Chemistry. In this study, we investigated the effect of positive dielectrophoresis (DEP) on gene expression in mesenchymal stem cells. When applying an alternating current voltage, human bone marrow derived mesenchym...
Satoshi Arimoto, Ken Shimono, Tomoyuki Yasukawa, Fumio Mizutani, Toshihiko Yoshioka
Analytical Sciences   32(4) 469-472   Jan 2016   [Refereed]
© The Japan Society for Analytical Chemistry. We report on a biosensor for cocaine based on the conformation change of DNA aptamer by capturing the cocaine molecules. The oxidation current of ferrocene conjugated on the terminal end of aptamer imm...
Tomoyuki Yasukawa, Yuya Yamashita, Riku Moede, Daichi Nakayama, Seiichiro Iijima, Fumio Mizutani
Analyst   140 1014-1018   Feb 2015   [Refereed]
© The Royal Society of Chemistry 2015. We report a novel electrochemical sensing system for single-stranded DNA (ssDNA) with a specific sequence based on the catalytic reduction of protons with platinum deposited by the electrochemical reduction o...
Yuki Igaki, Fumio Mizutani, Tomoyuki Yasukawa
Chemistry Letters   44 1031-1032   Jan 2015   [Refereed]
© 2015 The Chemical Society of Japan. The oxygen consumption of single contractile myotubes was investigated by amperometric monitoring of the reduction current for oxygen using scanning electrochemical microscope (SECM) equipped with Pt microelec...
Tomoyuki Yasukawa, Riku Moede, Fumio Mizutani
Sensors and Materials   27 817-823   Jan 2015   [Refereed]
© MYU K.K. An electrochemical detection system for DNA has been investigated based on the current increase due to the catalytic oxidation of hydrogen peroxide with platinum deposited by the electrochemical reduction of chloro-2,2':6',2''-terpyridi...
Tomoyuki Yasukawa, Yuya Kiba, Fumio Mizutani
Analytical Sciences   31 583-589   Jan 2015   [Refereed]
© 2015 The Japan Society for Analytical Chemistry. A dual-electrochemical sensor based on a test-strip assay with immunochemistry and enzyme reactions has been developed for the determination of albumin and creatinine. Each nitrocellulose membrane...
Tomoyuki Yasukawa, Yuya Kiba, Fumio Mizutani
Bunseki Kagaku   64 99-104   Jan 2015   [Refereed]
© 2015 The Japan Society for Analytical Chemistry. We fabricated an electrochemical detection system involving redox species flowing in a nitrocellulose membrane incorporated into two plates with both poles and electrodes. We prepared an upper sub...
Takuma Horii, Masashi Yamamoto, Tomoyuki Yasukawa, Tomoyuki Yasukawa, Fumio Mizutani
Biosensors and Bioelectronics   61 215-221   Nov 2014   [Refereed]
A rapid and simple method for the fabrication of the island patterns with particles and cells was applied to detect the presence of specific antigens on the cell surface. An upper interdigitated microband array (IDA) electrode was mounted on a low...
Yuki Yoshimura, Masahiro Tomita, Fumio Mizutani, Tomoyuki Yasukawa
Analytical Chemistry   86 6818-6822   Jul 2014   [Refereed]
We report a simple device with an array of 10 000 (100 × 100) microwells for producing vertical pairs of cells in individual microwells with a rapid manipulation based on positive dielectrophoresis (p-DEP). The areas encircled with micropoles whic...
Tomoyuki Yasukawa, Masahiro Koide, Norihisa Tatarazako, Ryoko Abe, Hitoshi Shiku, Fumio Mizutani, Tomokazu Matsue, Tomokazu Matsue
Analytical Chemistry   86 304-307   Jan 2014   [Refereed]
A novel measurement system to determine oxygen consumption rates via respiration in migrating Zebrafish (Danio rerio) has been developed. A signal equalization system was adapted to detect oxygen in a chamber with one fish, because typical electro...
Yuki Yoshimura, Chiaki Fujii, Masahiro Tomita, Fumio Mizutani, Tomoyuki Yasukawa
Chemistry Letters   43 980-981   Jan 2014   [Refereed]
We developed a simple device with 400 (20 × 20) microwells that integrated the cell positioning and cell pairing of two different types of cells based on positive dielectrophoretic manipulation. We fabricated a star-shaped micropole array on a con...
Toshiki Hokuto, Tomoyuki Yasukawa, Ryota Kunikata, Atsushi Suda, Kumi Y. Inoue, Kumi Y. Inoue, Tomokazu Matsue, Tomokazu Matsue, Tomokazu Matsue, Fumio Mizutani
Chemistry Letters   43 758-759   Jan 2014   [Refereed]
The activities of glucose oxidase (GOx) and lactate oxidase (LOx) immobilized on a substrate were obtained as images of oxidation currents of H2O2using a Bio-LSI system. In the presence of appropriate substrates, the image of the enzyme appeared a...
Tomoyuki Yasukawa, Tomoyuki Yasukawa, Junko Yamada, Hitoshi Shiku, Fumio Mizutani, Tomokazu Matsue
Sensors and Actuators, B: Chemical   186 9-16   Jun 2013   [Refereed]
Control of the flow position of cells in a microchannel is useful for developing cell separation systems. We demonstrated that cells with different sizes were transported through different gaps by a repulsive force generated by negative dielectrop...
Tomoyuki Yasukawa, Yusuke Yoshida, Hironobu Hatanaka, Fumio Mizutani
Journal of Robotics and Mechatronics   25 650-656   Jun 2013   [Refereed]
We report on control of line pattern positioning with particles fabricated by negative dielectrophoresis (n- DEP) using the applied intensity and phase of an AC electric field. Line patterns were fabricated in a microfluidic device consisting of u...
菊池美賀, 安川智之, 水谷文雄
表面技術   64(3) 190-192   Mar 2013   [Refereed]
Satoshi Arimoto, Akihito Kamei, Tomoyuki Yasukawa, Fumio Mizutani, Toshihiko Yoshioka
Electrochimica Acta   108 776-780   Jan 2013   [Refereed]
Based on the substitutional stripping voltammetry (SSV) that is a highly sensitive electrochemical mea-surement, we have developed a new measurement system employing a gel made from hydrophobic ionicliquid and polymer. Since a salt bridge and an a...
YASUKAWA Tomoyuki, YAMADA Junko, SHIKU Hitoshi, MIZUTANI Fumio, MATSUE Tomokazu
Journal of The Surface Finishing Society of Japan   64(1) 52-56   Jan 2013   [Refereed]
Antibody spots were used to demonstrate that cells with a specific antigen on the membrane can be captured selectively and simply. By dropping a few microliters of solution containing antibody, antibody spots were prepared on three substrates: gla...
Masashi Yamamoto, Tomoyuki Yasukawa, Tomoyuki Yasukawa, Masato Suzuki, Satoshi Kosuge, Hitoshi Shiku, Tomokazu Matsue, Fumio Mizutani
Electrochimica Acta   82 35-42   Nov 2012   [Refereed]
Island organization of particles have been fabricated in a microfluidic device consisted of upper and lower conductive indium-tin-oxide (ITO) substrates with interdigitated microband array (IDA) electrode used as the template based on a dielectrop...
Yuya Kiba, Yukako Otani, Tomoyuki Yasukawa, Tomoyuki Yasukawa, Fumio Mizutani
Electrochimica Acta   81 14-19   Oct 2012   [Refereed]
We have demonstrated the electrochemistry of a redox active species, Fe(CN)64-, flowing in a nitrocellulose membrane incorporated into two plates with a pole array and electrodes, and applied it by measuring the activity of an enzyme, glucose oxid...
Tomoyuki Yasukawa, Tomoyuki Yasukawa, Hironobu Hatanaka, Fumio Mizutani
Analytical Chemistry   84 8830-8836   Oct 2012   [Refereed]
We report the fabrication of two different cell patterns based on negative dielectrophoresis (n-DEP) and apply it to simple and rapid distinction of cells with specific surface antigens from a cell population. The DEP device for cell manipulation ...
Tomoyuki Yasukawa, Tomoyuki Yasukawa, Yoshimi Yoshimoto, Takuya Goto, Fumio Mizutani
Biosensors and Bioelectronics   37 19-23   Aug 2012   [Refereed]
In this work, a novel immunosensing method has been developed on the basis of the sensitive determination of a product generated by an enzyme reaction with dual amplification system combining an electrochemical-redox cycling and coulometric signal...
Masahiro Koide, Masahiro Koide, Tomoyuki Yasukawa, Yoshiko Horiguchi, Kuniaki Nagamine, Hitoshi Shiku, Tomokazu Matsue, Tomoaki Itayama, Tomoaki Itayama
Analytical Sciences   28 69-72   Mar 2012   [Refereed]
A microfluidic device with analytical chambers for electrochemical measurements has been employed to detect photosynthetic activity at single cell level. The flowing cells (Microcystis viridis) in a main channel are individually guided to the cham...
安川 智之, 末永 智一, 水谷 文雄
化学工業   63(3) 188-193   Mar 2012   [Refereed][Invited]
Yu Hirano, Tomoyuki Yasukawa, Yoshiaki Mase, Daisuke Oyamatsu, Hitoshi Shiku, Fumio Mizutani, Tomokazu Matsuec
Electrochemistry   80 30-32   Jan 2012   [Refereed]
Detectable sensitivity for immobilized enzymes was improved using a constant-distance mode of scanning electrochemical microscopy (SECM). Redox species generated by an enzyme reaction captured via irmmunorecognition were detected with a microelect...
Tomoyuki Yasukawa, Tomoyuki Yasukawa, Junko Yamada, Hitoshi Shiku, Fumio Mizutani, Tomokazu Matsue
Intelligent Automation and Soft Computing   18 201-211   Jan 2012   [Refereed]
In this work, we report the control of a microparticle position within fluid flow based on its size by using a repulsive force generated with negative dielectrophoresis (n-DEP). The n-DEP based fluidic channel, which was consisted of navigator and...
Tomoyuki Yasukawa, Tomoyuki Yasukawa, Yuki Yoshimura, Fumio Mizutani
Electrochemistry   80 15-17   Jan 2012   [Refereed]
We describe an improvement of the detectable range of glucose oxidase (GOx)-based microsensors to higher concentrations. Oxygen consumed by the enzyme reaction of GOx was detected cathodically by the microelectrode modified with poly(dimethylsilox...
Koide Masahiro, Yasukawa Tomoyuki, Horiguchi Yoshiko, Nagamine Kuniaki, Shiku Hitoshi, Matsue Tomokazu, Itayama Tomoaki
ANALYTICAL SCIENCES   28(1) 69-72   Jan 2012   [Refereed]
Javier Ramón-Azcón, Tomoyuki Yasukawa, Tomoyuki Yasukawa, Fumio Mizutani
Biosensors and Bioelectronics   28 443-449   Oct 2011   [Refereed]
In this study, a rapid immunosensing system has been developed for simultaneous analysis of two tumor markers, alpha-fetoprotein (AFP) and prostate-specific antigen (PSA). The strategy for rapid multisensing is based on rapid immunoreactions occur...
Yasufumi Takahashi, Takeshi Miyamoto, Hitoshi Shiku, Kosuke Ino, Tomoyuki Yasukawa, Ryutaro Asano, Izumi Kumagai, Tomokazu Matsue, Tomokazu Matsue
Physical Chemistry Chemical Physics   13 16569-16573   Oct 2011   [Refereed]
We report a scanning electrochemical microscopy (SECM)-based receptor-mediated endocytosis detection method. Epidermal growth factor receptor (EGFR), which is one of the key membrane proteins associated with cancer, was used as a model for recepto...
Hironobu Hatanaka, Tomoyuki Yasukawa, Tomoyuki Yasukawa, Fumio Mizutani
Analytical Chemistry   83 7207-7212   Sep 2011   [Refereed]
Rapid determination of surface antigens on cells is possible by immobilization of cells accumulated by positive dielectrophoresis (p-DEP) via effective surface immunoreactions and removal of unbound cells by negative DEP (n-DEP). The DEP device fo...
Masahiro Koide, Masahiro Koide, Tomoyuki Yasukawa, Kuniaki Nagamine, Hitoshi Shiku, Tomoaki Itayama, Tomokazu Matsue
Sensors and Actuators, B: Chemical   153 474-478   Apr 2011   [Refereed]
Electrochemical devices have been fabricated with 2 platinum (Pt) microelectrodes located at the bottom of microwells to characterize the photosynthetic activity of a single Microcystis viridis (cyanobacterium) cell. The cell was manipulated into ...
Kosuke Ino, Atsuko Ishida, Kumi Y. Inoue, Masato Suzuki, Masahiro Koide, Masahiro Koide, Tomoyuki Yasukawa, Hitoshi Shiku, Tomokazu Matsue
Sensors and Actuators, B: Chemical   153 468-473   Apr 2011   [Refereed]
We have developed an electrorotation (ER) chip that has a sandwich structure in which interdigitated array (IDA) electrodes are arranged face-to-face. These IDA electrodes on the top and bottom of the chip were orthogonally arranged to form over 2...
Javier Ramón-Azcón, Tomoyuki Yasukawa, Tomoyuki Yasukawa, Fumio Mizutani
Analytical Chemistry   83 1053-1060   Feb 2011   [Refereed]
In this work, we designed a new immunodevice that combines competitive immunoreactions on the microparticles, accumulation of these particles by negative dielectrophoresis (n-DEP), and their subsequent capture through hybridization among single-st...
Takuya Goto, Tomoyuki Yasukawa, Tomoyuki Yasukawa, Kazuhiro Kanda, Shinji Matsui, Fumio Mizutani
Analytical Sciences   27 91-94   Jan 2011   [Refereed]
An anti-fouling ability of diamond-like carbon (DLC) electrodes to biological macromolecules has been investigated from a decrease in the electrochemical redox current of Fe(CN) 6 4-/3-, used as a redox marker. A DLC electro...
OTANI Yukako, YASUKAWA Tomoyuki, MIZUTANI Fumio
BUNSEKI KAGAKU   59(8) 721-725   Aug 2010   [Refereed]
In this work, we have developed glucose biosensors based on chemical amplification with glucose cycling between glucose oxidase (GOx) and glucose dehydrogenase (GDH) immobilized on a poly(dimethylsiloxane) (PDMS) layer. PDMS-coated p...
Tomoyuki Yasukawa, Fumio Mizutani, Tomokazu Matsue
Journal of Japan Institute of Electronics Packaging   13(3) 188-193   May 2010   [Refereed][Invited]
Tomoyuki Yasukawa, Tomoyuki Yasukawa, Keisuke Goto, Fumio Mizutani
Electroanalysis   22 927-930   May 2010   [Refereed]
We report determination of the apparent Michaelis constant of glucose oxidase (GOx) immobilized on a microelectrode with respect to oxygen. We used a GOx-modified microelectrode as a probe for scanning electrochemical microscopy. We detected hydro...
Javier Ramón-Azcón, Tomoyuki Yasukawa, Tomoyuki Yasukawa, Hyun Jung Lee, Tomokazu Matsue, Francisco Sánchez-Baeza, Maria Pilar Marco, Fumio Mizutani
Biosensors and Bioelectronics   25 1928-1933   Apr 2010   [Refereed]
In this work, we have applied particle manipulation based on negative dielectrophoresis (n-DEP) to develop rapid and separation-free immunosensing systems. Two widely used pesticides, atrazine and bromopropylate, were used as target molecules to d...
Hyun Jung Lee, Sang Hyun Lee, Tomoyuki Yasukawa, Tomoyuki Yasukawa, Javier Ramón-Azcón, Fumio Mizutani, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue
Talanta   81 657-663   Apr 2010   [Refereed]
We report here a rapid, simple, and simultaneous immunosensing method for two tumor markers, alpha-fetoprotein (AFP) and carcinoembryonic antigen (CEA), by applying the negative-dielectrophoretic (n-DEP) manipulation of microparticles. Micropartic...
Kumi Y. Inoue, Kosuke Ino, Hitoshi Shiku, Shigenobu Kasai, Tomoyuki Yasukawa, Fumio Mizutani, Tomokazu Matsue
Biosensors and Bioelectronics   25 1723-1728   Mar 2010   [Refereed]
We developed an electrochemical-sensing device for continuous monitoring extracellular hydrogen peroxide (H2O2). The device consists of an indium-tin-oxide electrode coated with osmiumpolyvinylpyridine gel polymer containing horseradish peroxidase...
Tomoyuki Yasukawa, Tomoyuki Yasukawa, Shingo Inadumi, Rie Harada, Shingo Shinagawa, Hiroshi Nose, Fumio Mizutani
Chemistry Letters   39 88-89   Feb 2010   [Refereed]
A novel immunosensor is presented based on a charge accumulation system using an electrode modified with a polymer containing [osmium(4,4′-dimethyl-2,2′-bipyridine) 2chloride] +/2+ and horseradish peroxidase (Os/HRP polymer) to detect N 1,N 12-dia...
SHIKU Hitoshi, KUMAGAI Ayako, LUO Hong Qun, TAKAHASHI Yasufumi, YASUKAWA Tomoyuki, YAMADA Hiroshi, MATSUE Tomokazu
Electrochemistry   78(2) 122-125   Feb 2010   [Refereed]
Yu Hirano, Tomoyuki Yasukawa, Yoshihiro Sawayashiki, Hitoshi Shiku, Fumio Mizutani, Tomokazu Matsue
Electrochemistry   78 175-177   Jan 2010   [Refereed]
We prepared a microfluidic device with an interdigitated array electrode modified with antibodies to develop immunosensors. A comb-type array (W1) was used for forming immunocomplexes and another (W2) for detecting mediators generated by the enzym...
Yasufumi Takahashi, Yumi Murakami, Kuniaki Nagamine, Hitoshi Shiku, Shigeo Aoyagi, Tomoyuki Yasukawa, Makoto Kanzaki, Tomokazu Matsue
Physical Chemistry Chemical Physics   12 10012-10017   Jan 2010   [Refereed]
Scanning ion conductance microscopy (SICM) using a nanopipette as a probe and ionic current as a feedback signal was introduced as a novel technique to study live cells in a physiological environment. To avoid contact between the pipette tip and c...
Hitoshi Shiku, Ayako Kumagai, Hong Qun Luo, Yasufumi Takahashi, Tomoyuki Yasukawa, Hiroshi Yamada, Tomokazu Matsue
Electrochemistry   78 122-125   Jan 2010   [Refereed]
Microcontact printing (μCP) with various proteins has been widely applied to biosensors and cell biology research. However, the mechanism of protein patterning in the μCP process is not clear in detail We have electrochemically estimated enzyme co...
Tatsuya Murata, Tomoyuki Yasukawa, Hitoshi Shiku, Tomokazu Matsue
Biosensors and Bioelectronics   25 913-919   Dec 2009   [Refereed]
Scanning electrochemical microscopy (SECM) was used for the analysis of single-cell gene-expression signals on the basis of a reporter system. We microfabricated a single-cell array on an Indium tin oxide (ITO) electrode comprising 4 × 4 SU-8 micr...
Kosuke Ino, Yusuke Kitagawa, Tsuyoshi Watanabe, Hitoshi Shiku, Masahiro Koide, Masahiro Koide, Tomoaki Itayama, Tomoyuki Yasukawa, Tomokazu Matsue
Electrophoresis   30 3406-3412   Dec 2009   [Refereed]
Endocrine disruptors that act like hormones in the endocrine system might have toxic effects. Therefore, it is important to develop a portable device that can detect hormone active chemicals in samples rapidly and easily. In this study, a microflu...
Yasufumi Takahashi, Hitoshi Shiku, Tatsuya Murata, Tomoyuki Yasukawa, Tomokazu Matsue
Analytical Chemistry   81 9674-9681   Dec 2009   [Refereed]
Gene-transfected single HeLa cells were characterized using a scanning electrochemical/optical microscope (SECM/OM) system with shear-force-based probe-sample distance regulation to simultaneously capture electrochemical, fluorescent, and topograp...
Kosuke Ino, Hitoshi Shiku, Fumisato Ozawa, Tomoyuki Yasukawa, Tomokazu Matsue
Biotechnology and Bioengineering   104 709-718   Nov 2009   [Refereed]
In this study, a useful method was developed to fabricate array patterns of microparticles not on electrode surfaces, but on arbitrary surfaces, using negative-dielectrophoresis (n-DEP). First, electrodes were designed and electric field simulatio...
Tomoyuki Yasukawa, Tomoyuki Yasukawa, Masato Suzuki, Hitoshi Shiku, Tomokazu Matsue
Sensors and Actuators, B: Chemical   142 400-403   Oct 2009   [Refereed]
We report here the control of the microparticles position within fluid flow based on its size by using dielectrophoresis (DEP) with a microelectrode array consisted of rectangular features with the different size of width and gap. 3 μm- and 10 μm-...
Yuki Yoshimoto, Tomoyuki Yasukawa, Tomoyuki Yasukawa, Fumio Mizutani
Analyst   134 2113-2117   Sep 2009   [Refereed]
A novel electrochemical technique for DNA detection has been developed by a simple combination cisplatin and a glassy carbon electrode. The complexation of cisplatin with DNA caused suppression of the electroreduction of cisplatin owing to the ste...
Tomoyuki Yasukawa, Tomoyuki Yasukawa, Eiji Maekawa, Fumio Mizutani
Analytical Sciences   25 1159-1162   Sep 2009   [Refereed]
Poly(dimethylsiloxane) (PDMS)-coated platinum electrodes modified with glucose oxidase (GOx) have been prepared from poly(L-lysine) (polymer backbone), glutaraldehyde (cross-linking agent) and poly(ethylene glycol) units. To fabricate a GOx layer ...
Ryouta Kunikata, Yasufumi Takahashi, Masahiro Koide, Masahiro Koide, Tomoaki Itayama, Tomoyuki Yasukawa, Hitoshi Shiku, Tomokazu Matsue
Sensors and Actuators, B: Chemical   141 256-262   Aug 2009   [Refereed]
We microfabricated a novel device consisting of a 4 × 4 array microchamber sandwiched with the two microband electrode array. This device allows dielectrophoretic (DEP) manipulation of microbeads to introduce into and release out a certain address...
YASUKAWA Tomoyuki, MAEKAWA Eiji, MIZUTANI Fumio
BUNSEKI KAGAKU   58(7) 639-644   Jul 2009   [Refereed]
The permeability for oxygen and hydrogen peroxide through a poly(dimethylsiloxane) (PDMS) layer with various thicknesses on a platinum electrode was investigated in order to develop a glucose sensor based on the detection of a reduction current of...
Yasufumi Takahashi, Yasufumi Takahashi, Yasufumi Takahashi, Takeshi Miyamoto, Takeshi Miyamoto, Takeshi Miyamoto, Hitoshi Shiku, Hitoshi Shiku, Hitoshi Shiku, Ryutaro Asano, Ryutaro Asano, Ryutaro Asano, Tomoyuki Yasukawa, Tomoyuki Yasukawa, Tomoyuki Yasukawa, Izumi Kumagai, Izumi Kumagai, Izumi Kumagai, Tomokazu Matsue, Tomokazu Matsue, Tomokazu Matsue
Analytical Chemistry   81 2785-2790   Apr 2009   [Refereed]
A membrane protein on the surface of a single living mammalian cell was imaged by scanning electrochemical microscopy (SECM). The epidermal growth factor receptor (EGFR) is one of the key membrane proteins associated with cancer. It elicits a wide...
Javier Ramón-Azcón, Ryouta Kunikata, F. J. Sanchez, M. P. Marco, Hitoshi Shiku, Tomoyuki Yasukawa, Tomokazu Matsue
Biosensors and Bioelectronics   24 1592-1597   Feb 2009   [Refereed]
The detection of atrazine using a novel optical immunosensing technique based on negative dielectrophoresis (n-DEP) in microfluidic channels is described. Atrazine is a toxic triazine herbicide within the most frequently used. Polystyrene micropar...
Hitoshi Shiku, Takeshi Yamakawa, Yuji Nashimoto, Yasufumi Takahashi, Yu suke Torisawa, Tomoyuki Yasukawa, Takahiro Ito-Sasaki, Masaki Yokoo, Hiroyuki Abe, Hideki Kambara, Hideki Kambara, Tomokazu Matsue
Analytical Biochemistry   385 138-142   Feb 2009   [Refereed]
Collection of bioanalytes from single cells is still a challenging technology despite the recent progress in many integrated microfluidic devices. A microfluidic dual capillary probe was prepared from a theta (θ)-shaped glass capillary to analyze ...
Hitoshi Shiku, Shun Goto, Sungbong Jung, Kuniaki Nagamine, Masahiro Koide, Tomosato Itayama, Tomoyuki Yasukawa, Tomokazu Matsue
Analyst   134 182-187   Jan 2009   [Refereed]
A highly sensitive and quantitative analysis was performed using a poly(dimethylsiloxane) (PDMS) microwell array in a scanning electrochemical microscopy setup. A microelectrode with a relatively large seal radius was used to cover the top of the ...
Eiji Maekawa, Nao Kitano, Tomoyuki Yasukawa, Fumio Mizutani
Electrochemistry   77 319-321   Jan 2009   [Refereed]
A glucose-sensing electrode based on the cathodic detection of oxygen, which was consumed with the glucose oxidase-catalyzed reaction in the presence of glucose, was prepared by immobilizing the enzyme on a surface-modified poly(dimethylsiloxane) ...
Hyun Jung Lee, Tomoyuki Yasukawa, Masato Suzuki, Sang Hyun Lee, Takafumi Yao, Yusuke Taki, Akira Tanaka, Masaomi Kameyama, Hitoshi Shiku, Tomokazu Matsue
Sensors and Actuators, B: Chemical   136 320-325   Jan 2009   [Refereed]
Arrays of gold nanoparticles and gold nanorods have been fabricated in the spatially localized pores of a porous alumina membrane using positive dielectrophoresis (p-DEP). The porous alumina membrane with a pore size of 200 nm and a thickness of 6...
Fumio Mizutani, Eiji Ohta, Yasuhiro Mie, Osamu Niwa, Tomoyuki Yasukawa
Sensors and Actuators, B: Chemical   135 304-308   Dec 2008   [Refereed]
A novel electrochemical enzyme immunoassay system with a detection limit of 2 pM (10 ng L-1) was developed for the determination of insulin, an important hormone for clinical diagnosis of diabetes. An insulin-containing sample solution was added t...
Masato Suzuki, Tomoyuki Yasukawa, Hitoshi Shiku, Tomokazu Matsue
Biosensors and Bioelectronics   24 1043-1047   Dec 2008   [Refereed]
In this paper, a novel method for patterning different cell types based on negative dielectrophoresis (n-DEP), without any special pretreatment of a culture slide, has been described. An interdigitated array (IDA) electrode with four independent m...
YASUKAWA Tomoyuki, SHUKU Hitoshi, MIZUTANI Fumio, MATSUE Tomokazu
Journal of The Surface Finishing Society of Japan   59(12) 818-824   Dec 2008   [Refereed][Invited]
Hyun Jung Lee, Tomoyuki Yasukawa, Hitoshi Shiku, Tomokazu Matsue
Biosensors and Bioelectronics   24 1000-1005   Dec 2008   [Refereed]
We report here a rapid and separation-free immunoassay using a dielectrophoresis (DEP) device consisting of an interdigitated microarray (IDA) electrode and a polydimethylsiloxane (PDMS) substrate. On applying an AC voltage to the IDA in a negativ...
Tomoyuki Yasukawa, Tomoyuki Yasukawa, Kuniaki Nagamine, Yoshiko Horiguchi, Hitoshi Shiku, Masahiro Koide, Tomoaki Itayama, Fujio Shiraishi, Tomokazu Matsue, Tomokazu Matsue
Analytical Chemistry   80 3722-3727   May 2008   [Refereed]
A novel microfluidic device with an array of analytical chambers was developed in order to perform single-cell-based gene-function analysis. A series of analytical processes was carried out using the device, including electrophoretic manipulation ...
Hyun Jung Lee, Tomoyuki Yasukawa, Masato Suzuki, Yusuke Taki, Akira Tanaka, Masaomi Kameyama, Hitoshi Shiku, Tomokazu Matsue
Sensors and Actuators, B: Chemical   131 424-431   May 2008   [Refereed]
We report here a rapid formation of island arrays with nanoparticles on and within polycarbonate (PC) membrane based on positive dielectrophoresis (p-DEP). For the fabrication of the patterning device, PC membranes with 10 μm thickness and 100, 20...
H. Shiku, K. Nagamine, T. Kaya, T. Yasukawa, T. Matsue
Bioelectrochemistry: Fundamentals, Experimental Techniques and Applications   249-266   Apr 2008
Kumi Y. Inoue, Tomoyuki Yasukawa, Tomoyuki Yasukawa, Hitoshi Shiku, Tomokazu Matsue
Electrochemistry   76 525-528   Jan 2008   [Refereed]
We developed a cell-based assay device for the detection of endotoxin, the potentially toxic compound that induces septic shock. Genetically-engineered cells that secrete alkaline phosphatase (SEAP) on exposure to endotoxin were cultured in an ele...
Hong Qun Luo, Hong Qun Luo, Hitoshi Shiku, Ayako Kumagai, Yasufumi Takahashi, Tomoyuki Yasukawa, Tomokazu Matsue
Electrochemistry Communications   9 2703-2708   Nov 2007   [Refereed]
Micropatterns of diaphorase (Dp) were fabricated on glass substrates by the microcontact printing (μCP) method and characterized with atomic force microscopy (AFM) and scanning electrochemical microscopy (SECM). AFM images of the printed samples r...
Kumi Inoue, Pascal Ferrante, Yu Hirano, Tomoyuki Yasukawa, Hitoshi Shiku, Tomokazu Matsue
Talanta   73 886-892   Oct 2007   [Refereed]
An immunochromatographic assay using nitrocellulose membrane was combined with electrochemical detection using an electrode chip in order to quantitatively detect testosterone as a model analyte. The electrode chip consisted of a gold working elec...
Yuji Nashimoto, Yasufumi Takahashi, Takeshi Yamakawa, Yu Suke Torisawa, Tomoyuki Yasukawa, Takahiro Ito-Sasaki, Masaki Yokoo, Hiroyuki Abe, Hitoshi Shiku, Hideki Kambara, Hideki Kambara, Tomokazu Matsue, Tomokazu Matsue
Analytical Chemistry   79 6823-6830   Sep 2007   [Refereed]
The cytosol of a single adherent cell was collected by the electrical cell lysis method with a Pt-ring capillary probe, and the cellular messenger RNA (mRNA) was analyzed at a single-cell level. The ring electrode probe was positioned 20 μm above ...
Ching Chou Wu, Takeshi Saito, Tomoyuki Yasukawa, Hitoshi Shiku, Hiroyuki Abe, Hiroyoshi Hoshi, Tomokazu Matsue
Sensors and Actuators, B: Chemical   125 680-687   Aug 2007   [Refereed]
The detection of oxygen consumption as an indicator of the bovine embryo activity has attracted much attention. A microfluidic chip with the built-in amperometric detector array was successfully constructed to transport, immobilize, and in situ me...
Hiroyuki Nakano, Hiroyuki Nakano, Kaoru Dokko, Kaoru Dokko, Jun ichi Sugaya, Tomoyuki Yasukawa, Tomokazu Matsue, Kiyoshi Kanamura, Kiyoshi Kanamura
Electrochemistry Communications   9 2013-2017   Aug 2007   [Refereed]
Micro-batteries were fabricated by using BAB block copolymer as dry polymer electrolyte, which consisted of polyethylene oxide and polystyrene and had relatively high ionic conductivity at room temperature. The micro-batteries were fabricated by a...
Yu Suke Torisawa, Yuji Nashimoto, Tomoyuki Yasukawa, Hitoshi Shiku, Tomokazu Matsue
Biotechnology and Bioengineering   97 615-621   Jun 2007   [Refereed]
Three cell lines, that is, the human breast cancer cell line (MCF-7) and the human mammary epithelial cell line (S-1) and its malignant form (T4-2) were embedded in a reconstituted basement membrane (Matrigel) that had 20-nL pyramid-shaped silicon...
Tomoyuki Yasukawa, Yu Hirano, Naomi Motochi, Hitoshi Shiku, Tomokazu Matsue
Biosensors and Bioelectronics   22 3099-3104   Jun 2007   [Refereed]
Scanning electrochemical microscopy (SECM) was applied to a dual enzyme immunoassay for the detection of pepsinogen 1 (PG1) and pepsinogen 2 (PG2). Sandwich-type immunocomplexes labeled with horseradish peroxidase (HRP) were constructed on microsp...
INOUE Kumi, FERRANTE Pascal, HIRANO Yu, YASUKAWA Tomoyuki, SHIKU Hitoshi, MATSUE Tomokazu
BUNSEKI KAGAKU   56(6) 471-478   Jun 2007   [Refereed]
A quantitative electrochemical detection of testosterone was performed using a nitrocellulose membrane as a support for antibodies. Testosterone competitively reacted with horseradish peroxidase (HRP)-labeled testosterone, and was captured with an...
Tomoyuki Yasukawa, Masato Suzuki, Takashi Sekiya, Hitoshi Shiku, Tomokazu Matsue
Biosensors and Bioelectronics   22 2730-2736   May 2007   [Refereed]
Microparticles have been manipulated in a microfluidic channel by means of negative dielectrophoresis (n-DEP), and the approach applied to a heterogeneous immunoassay system. A microfluidic device, with three-dimensional (3-D) microelectrodes fabr...
Kaoru Dokko, Kaoru Dokko, Jun ichi Sugaya, Hiroyuki Nakano, Tomoyuki Yasukawa, Tomokazu Matsue, Kiyoshi Kanamura, Kiyoshi Kanamura
Electrochemistry Communications   9 857-862   May 2007   [Refereed]
Microarray electrodes of LiMn2O4and Li4/3Ti5/3O4were prepared on a glass substrate using a sol-gel method. The prepared LiMn2O4and Li4/3Ti5/3O4microarray electrodes were characterized with scanning electron microscopy, Raman spectroscopy, and cycl...
Kuniaki Nagamine, Shiho Onodera, Ai Kurihara, Tomoyuki Yasukawa, Hitoshi Shiku, Ryutaro Asano, Izumi Kumagai, Tomokazu Matsue
Biotechnology and Bioengineering   96 1008-1013   Apr 2007   [Refereed]
A microbial array chip with collagen gel spots entrapping living Escherichia coli (E. coli) DH5α was applied for the screening of recombinant protein solubilities. The α-fragment of β-galactosidase (βGal) was fused to the target protein, namely, m...
Masato Suzuki, Tomoyuki Yasukawa, Hitoshi Shiku, Tomokazu Matsue
Langmuir   23 4088-4094   Mar 2007   [Refereed]
Microparticle patterns have been fabricated on a nonconductive glass substrate and a conductive indium tin oxide (ITO) substrate using negative dielectrophoresis (n-DEP). The patterned microparticles on the substrate were immobilized by covalent b...
Yu suke Torisawa, Airi Takagi, Yuji Nashimoto, Tomoyuki Yasukawa, Hitoshi Shiku, Tomokazu Matsue
Biomaterials   28 559-566   Jan 2007   [Refereed]
We describe a novel multicellular spheroid culture system that facilitates the easy preparation and culture of a spheroid microarray for the long-term monitoring of cellular activity. A spheroid culture device with an array of pyramid-like microho...
Yasufumi Takahashi, Yu Hirano, Tomoyuki Yasukawa, Hitoshi Shiku, Hiroshi Yamada, Tomokazu Matsue
Langmuir   22 10299-10306   Dec 2006   [Refereed]
We developed a high-resolution scanning electrochemical microscope (SECM) for the characterization of various biological materials. Electrode probes were fabricated by Ti/Pt sputtering followed by parylene C-vapor deposition polymerization on the ...
YASUKAWA Tomoyuki, HIRANO Yu, OGASAWARA Daichi, MOTOCHI Naomi, SHIKU Hitoshi, KAWABATA Shohei, MATSUE Tomokazu
BUNSEKI KAGAKU   55(12) 979-985   Dec 2006   [Refereed]
Scanning electrochemical microscopy (SECM) has been applied to enzyme immunoassay for the detection of C-reactive protein (CRP). The immunocomplexes of the sandwich type with horseradish peroxidase (HRP) labeling were constructed at the microspots...
Estimation of respiratory activity of mouse embryos by scanning electrochemical microscopy
Ito-Sasaki Takahiro, Okazaki Naoto, Yokoo Masaki, Shiku Hitoshi, Yasukawa Tomoyuki, Matsue Tomokazu, Abe Hiroyuki
ZOOLOGICAL SCIENCE   23(12) 1185   Dec 2006   [Refereed]
Yu Suke Torisawa, Noriko Ohara, Kuniaki Nagamine, Shigenobu Kasai, Tomoyuki Yasukawa, Hitoshi Shiku, Tomokazu Matsue
Analytical Chemistry   78 7625-7631   Nov 2006   [Refereed]
Electrochemical monitoring of cellular signal transduction under three-dimensional (3-D) cell culture conditions has been demonstrated by combining cell-based microarrays with a secreted alkaline phosphatase (SEAP) reporter system. The cells were ...
Sang Hyun Lee, Wook Hyun Lee, Seog Woo Lee, Hiroki Goto, Takeshi Baba, Meoung Whan Cho, Takafumi Yao, Takafumi Yao, Hyun Jung Lee, Tomoyuki Yasukawa, Tomokazu Matsue, Hyunchul Ko
Journal of Nanoscience and Nanotechnology   6 3351-3354   Nov 2006   [Refereed]
Vertically-aligned zinc oxide (ZnO) nano-needles have been selectively grown on the Si (100) substrates using chemical vapor transport and condensation method without metal catalyst. The selective nucleation of nano-needles was achieved by the con...
TAKAHASHI Yasufumi, SHIKU Hitoshi, YASUKAWA Tomoyuki, MATSUE Tomokazu
Journal of the Surface Science Society of Japan   27(10) 613-616   Oct 2006   [Refereed][Invited]
Takeshi Saito, Ching Chou Wu, Hitoshi Shiku, Tomoyuki Yasukawa, Masaki Yokoo, Takashi Ito-Sasaki, Hiroyuki Abe, Hiroyoshi Hoshi, Tomokazu Matsue, Tomokazu Matsue
Analyst   131 1006-1011   Sep 2006   [Refereed]
A quantitative analysis of the oxygen concentration profile near a poly(dimethylsiloxane) (PDMS) microfluidic device was performed using scanning electrochemical microscopy (SECM). A microchannel filled with sodium sulfite (Na2SO3) aqueous solutio...
Shigenobu Kasai, Hitoshi Shiku, Yu suke Torisawa, Kuniaki Nagamine, Tomoyuki Yasukawa, Toshiaki Watanabe, Tomokazu Matsue
Analytica Chimica Acta   566 55-59   Apr 2006   [Refereed]
A novel cytokine assay has been designed using a cellular chip by combining a collagen gel embedded cell culture technique with scanning electrochemical microscopy-enzyme linked immunosorbent assay (SECM-ELISA). An array of cell-collagen gel mixtu...
Daichi Ogasawara, Yu Hirano, Tomoyuki Yasukawa, Hitoshi Shiku, Kiichirou Kobori, Koji Ushizawa, Shohei Kawabata, Tomokazu Matsue
Biosensors and Bioelectronics   21 1784-1790   Mar 2006   [Refereed]
An electrochemical microdevice with separable electrode and antibody chips has been developed and applied to detect atrophic gastritis-related proteins, pepsinogen 1 (PG1) and pepsinogen 2 (PG2), based on sandwich-type enzyme-linked immunosorbent ...
Sang Hyun Lee, Hyun Jung Lee, Dongcheol Oh, Seog Woo Lee, Hiroki Goto, Ryan Buckmaster, Tomoyuki Yasukawa, Tomokazu Matsue, Soon Ku Hong, Hyunchui Ko, Meoung Whan Cho, Meoung Whan Cho, Takafumi Yao, Takafumi Yao
Journal of Physical Chemistry B   110 3856-3859   Mar 2006   [Refereed]
We report on the growth of uniquely shaped ZnO nanowires with high surface area and patterned over large areas by using a poly(dimethylsiloxane) (PDMS) microfluidic channel technique. The synthesis uses first a patterned seed template fabricated b...
Hitoshi Shiku, Takeshi Saito, Ching Chou Wu, Tomoyuki Yasukawa, Masaki Yokoo, Hiroyuki Abe, Tomokazu Matsue, Tomokazu Matsue, Hiroshi Yamada
Chemistry Letters   35 234-235   Feb 2006   [Refereed]
Scanning electrochemical microscopy (SECM) was employed to quantitatively characterize the oxygen permeation behaviors of poly(dimethylsiloxane) (PDMS) and surface-modified PDMS. The mass-transfer process of oxygen from the PDMS substrate to the t...

Misc

 
Tomoyuki Yasukawa, Fumio Mizutani
2017 6th International Conference on Informatics, Electronics and Vision and 2017 7th International Symposium in Computational Medical and Health Technology, ICIEV-ISCMHT 2017   2018-January 1-2   Apr 2018
© 2017 IEEE. The handling of individual cells is important for investigating characteristics of cells. Dielectrophoresis (DEP) by the microelectrode have been applied to manipulate single target cells as a micro-tweezer [1-2]. A target cell was tr...

Books etc

 
Hyper Bio Assembler for 3D Cellular Systems
Tomoyuki Yasukawa, Fumio Mizutani (Part:Contributor, Chapter 5, Discrimination of Cells with Specific Antigens Expressed on a Membrane Based on the Dielectrophoresis)
Springer   2015   
Biosensors and Environment Health
Javier Ramón-Azcón,Tomoyuki Yasukawa, Fumio Mizutani (Part:Contributor, Detection of Pesticide Residues using Biosensors)
CRC press   2012   
“Whole-cell biosensors”, in Bioelectrochemistry: fundamentals, experimental techniques, and applications
H. Shiku, K. Nagamine, T. Kaya, T. Yasukawa, T. Matsue (Part:Contributor, Chapter 7)
John Wiley & Sons Ltd.   Apr 2008   

Conference Activities & Talks

 
鈴木雅登, 寺尾和輝, 國方亮太, 須田篤史, 井上(安田)久美, 伊野浩介, 末永智一, 安川智之
日本分析化学会年会講演要旨集   29 Aug 2018   
井ノ崎玲央奈, 窪田慎太郎, 有本聡, 中山浩, 吉岡俊彦, 鈴木雅登, 安川智之
化学とマイクロ・ナノシステム学会研究会講演要旨集   21 May 2018   
岡山太樹, 鈴木雅登, 磯崎勇志, 湊元幹太, 冨田昌弘, 安川智之
化学とマイクロ・ナノシステム学会研究会講演要旨集   21 May 2018   
寺尾和輝, 鈴木雅登, 國方亮太, 須田篤史, 井上(安田)久美, 伊野浩介, 末永智一, 安川智之
化学とマイクロ・ナノシステム学会研究会講演要旨集   21 May 2018   
寺尾和輝, 國方亮太, 須田篤史, 井上(安田)久美, 伊野浩介, 末永智一, 安川智之
電気化学会大会講演要旨集(CD-ROM)   23 Feb 2018