論文

査読有り 国際誌
2020年9月

Nuclear staining of claudin-18 is a new immunohistochemical marker for diagnosing intramucosal well-differentiated gastric adenocarcinoma.

Pathology international
  • Yu Takahashi
  • ,
  • Yutaka Tsutsumi
  • ,
  • Chihiro Takeuchi
  • ,
  • Kazuya Shiogama
  • ,
  • Yasuyoshi Mizutani
  • ,
  • Ken-Ichi Inada
  • ,
  • Nobutake Yamamichi
  • ,
  • Kazuhiko Koike

70
9
開始ページ
644
終了ページ
652
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/pin.12978
出版者・発行元
Wiley

Diagnosis of gastric adenocarcinoma using small biopsy samples is occasionally difficult. Various markers have been employed for improving the diagnostic accuracy, but there remains room for improvement. A total of 129 endoscopically biopsied samples were studied, consisting of 104 intramucosal tubular adenocarcinomas, 24 non-cancerous lesions and one cancer sample originally suspected of non-cancer but revised as cancer after immunostaining. We evaluated the association between histopathology and immunohistochemical expression of MUC1, HER2, p53, CEA, E-cadherin, β-catenin and claudin-18. Regarding β-catenin and claudin-18, not only membranous expression (β-catenin(M) and claudin-18(M)) but also nuclear expression (β-catenin(N) and claudin-18(N)) were analyzed. When subtyped with mucin core protein expression, the gastric-type cancers dominantly expressed claudin-18(M), while claudin-18(N) was significantly encountered in intestinal- and mixed-types. Expression of MUC1 (P = 0.0010), HER2 (P = 0.0173), p53 (P = 0.0002), CEA (P = 0.0019) and claudin-18(N) (P < 0.0001) revealed significant correlation with gastric cancers. Negative correlation of claudin-18(M) (P = 0.0125) was also noted. MUC1 and p53 were negative in non-cancer lesions. The non-cancer group exceptionally expressed HER2 and β-catenin(N). Membranous expression of E-cadherin was consistent in both groups. Logistic regression analysis showed that MUC1 (P = 0.0086), p53 (P = 0.0031), claudin-18(M) (P = 0.0158) and claudin-18(N) (P = 0.0190) were independently associated with gastric cancers. Nuclear expression of claudin-18 should be the novel diagnostic marker for gastric cancer.

リンク情報
DOI
https://doi.org/10.1111/pin.12978
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32623829
URL
https://onlinelibrary.wiley.com/doi/pdf/10.1111/pin.12978
URL
https://onlinelibrary.wiley.com/doi/full-xml/10.1111/pin.12978

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