論文

査読有り
2018年

Cell-size confinement effect on protein diffusion in crowded poly(ethylene)glycol solution

Physical Chemistry Chemical Physics
  • Chiho Watanabe
  • ,
  • Miho Yanagisawa

20
13
開始ページ
8842
終了ページ
8847
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1039/c7cp08199e
出版者・発行元
Royal Society of Chemistry

Micrometric membrane confinements and macromolecular crowding of cytoplasm are key factors that regulate molecular diffusion in live cells. Previous studies have shown that macromolecular crowding delays molecular diffusion. However, the effect of cell-size confinement on diffusion in the crowding environment is yet to be elucidated. Using fluorescence correlation spectroscopy (FCS), we analyzed protein diffusion in microdroplets containing polymer solution covered with lipid membranes that mimic cells. As a result, we found that a synergistic condition of crowding and micrometric confinement results in accelerated protein diffusion on a sub-millisecond time scale. This acceleration rate strongly depended on the size of the confined space and the degree of crowding. These findings indicate that cell-size confinement supports protein diffusion in highly crowded cytoplasm.

Web of Science ® 被引用回数 : 5

リンク情報
DOI
https://doi.org/10.1039/c7cp08199e
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000428779700046&DestApp=WOS_CPL

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