論文

査読有り 国際誌
2016年6月

Upregulation of connexin43 contributes to PX-12-induced oxidative cell death.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine
  • Gang Li
  • ,
  • Kun Gao
  • ,
  • Yuan Chi
  • ,
  • Xiling Zhang
  • ,
  • Takahiko Mitsui
  • ,
  • Jian Yao
  • ,
  • Masayuki Takeda

37
6
開始ページ
7535
終了ページ
46
記述言語
英語
掲載種別
DOI
10.1007/s13277-015-4620-7

Thioredoxin (Trx) is a small redox protein that underlies aggressive tumor growth and resistance to chemotherapy. Inhibition of Trx with the chemical inhibitor PX-12 suppresses tumor growth and induces cell apoptosis. Currently, the mechanism underlying the therapeutic actions of PX-12 and the molecules influencing cell susceptibility to PX-12 are incompletely understood. Given that connexin43 (Cx43), a tumor suppressor, regulates tumor cell susceptibility to chemotherapy, we examined the possible involvement of Cx43 in PX-12-induced cell death. Exposure of cells to PX-12 led to a loss of cell viability, which was associated with the activation of oxidative sensitive c-Jun N-terminal kinase (JNK). Inhibition of JNK or supplement of cells with anti-oxidants prevented the cell-killing action of PX-12. The forced expression of Cx43 in normal and tumor cells increased cell sensitivity to PX-12-induced JNK activation and cell death. In contrast, the downregulation of Cx43 with siRNA or the suppression of gap junctions with chemical inhibitors attenuated JNK activation and enhanced cell resistance to PX-12. Further analysis revealed that PX-12 at low concentrations induced a JNK-dependent elevation in the Cx43 protein, which was also preventable by supplementing the cells with anti-oxidants. Our results thus indicate that Cx43 is a determinant in the regulation of cell susceptibility to PX-12 and that the upregulation of Cx43 may be an additional mechanism by which PX-12 exerts its anti-tumor actions.

リンク情報
DOI
https://doi.org/10.1007/s13277-015-4620-7
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/26684802
ID情報
  • DOI : 10.1007/s13277-015-4620-7
  • PubMed ID : 26684802

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