2010年2月
Selective Excitation of Photosystems in Chloroplasts Inside Plant Leaves Observed by Near-Infrared Laser-Based Fluorescence Spectral Microscopy
PLANT AND CELL PHYSIOLOGY
- ,
- ,
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- 巻
- 51
- 号
- 2
- 開始ページ
- 225
- 終了ページ
- 238
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1093/pcp/pcp182
- 出版者・発行元
- OXFORD UNIV PRESS
In this study, we produced selective images of photosystems in plant chloroplasts in situ. We used a spectroimaging microscope, equipped with a near-infrared (NIR) laser that provided light at wavelengths mainly between 800 and 830nm, to analyze chlorophyll autofluorescence spectra and images from chloroplasts in leaves of Zea mays at room temperature. Femtosecond laser excitation of chloroplasts in mesophyll cells revealed a spectral shape that was attributable to PSII and its antenna in the centers of grana spots. We found that a continuous wave emitted by the NIR laser at a wavelength as long as 820nm induced chlorophyll autofluorescence with a high contribution from PSI through a one-photon absorption mechanism. A spectral shape attributable to PSI and its antenna was thus obtained using continuous wave laser excitation of chloroplasts in bundle sheath cells. These highly pure spectra of photosystems were utilized for spectral decomposition at every intrachloroplast space to show distributions of PSI and PSII and their associated antenna. A new methodology using an NIR laser to detect the PSI/PSII ratio in single chloroplasts in leaves at room temperature is described.
- リンク情報
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- DOI
- https://doi.org/10.1093/pcp/pcp182
- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201002278194062975
- CiNii Articles
- http://ci.nii.ac.jp/naid/10027346696
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/20022977
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000274497300005&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1093/pcp/pcp182
- ISSN : 0032-0781
- J-Global ID : 201002278194062975
- CiNii Articles ID : 10027346696
- PubMed ID : 20022977
- Web of Science ID : WOS:000274497300005