論文

査読有り
2017年3月

The molecular basis of the organization of repetitive DNA-containing constitutive heterochromatin in mammals

CHROMOSOME RESEARCH
  • Gohei Nishibuchi
  • ,
  • Jerome Dejardin

25
1
開始ページ
77
終了ページ
87
記述言語
英語
掲載種別
DOI
10.1007/s10577-016-9547-3
出版者・発行元
SPRINGER

Constitutive heterochromatin is composed mainly of repetitive elements and represents the typical inert chromatin structure in eukaryotic cells. Approximately half of the mammalian genome is made of repeat sequences, such as satellite DNA, telomeric DNA, and transposable elements. As essential genes are not present in these regions, most of these repeat sequences were considered as junk DNA in the past. However, it is now clear that these regions are essential for chromosome stability and the silencing of neighboring genes. Genetic and biochemical studies have revealed that histone methylation at H3K9 and its recognition by heterochromatin protein 1 represent the fundamental mechanism by which heterochromatin forms. Although this molecular mechanism is highly conserved from yeast to human cells, its detailed epigenetic regulation is more complex and dynamic for each distinct constitutive heterochromatin structure in higher eukaryotes. It can also vary according to the developmental stage. Chromatin immunoprecipitation followed by sequencing (ChIP-seq) analysis is a powerful tool to investigate the epigenetic regulation of eukaryote genomes, but non-unique reads are usually discarded during standard ChIP-seq data alignment to reference genome databases. Therefore, specific methods to obtain global epigenetic information concerning repetitive elements are needed. In this review, we focus on such approaches and we summarize the latest molecular models for distinct constitutive heterochromatin types in mammals.

Web of Science ® 被引用回数 : 69

リンク情報
DOI
https://doi.org/10.1007/s10577-016-9547-3
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28078514
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000396339900008&DestApp=WOS_CPL
ID情報
  • DOI : 10.1007/s10577-016-9547-3
  • ISSN : 0967-3849
  • eISSN : 1573-6849
  • PubMed ID : 28078514
  • Web of Science ID : WOS:000396339900008

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