論文

査読有り
2014年10月

Physical and Functional Interactions between the Histone H3K4 Demethylase KDM5A and the Nucleosome Remodeling and Deacetylase (NuRD) Complex

JOURNAL OF BIOLOGICAL CHEMISTRY
  • Gohei Nishibuchi
  • ,
  • Yukimasa Shibata
  • ,
  • Tomohiro Hayakawa
  • ,
  • Noriyo Hayakawa
  • ,
  • Yasuko Ohtani
  • ,
  • Kaori Sinmyozu
  • ,
  • Hideaki Tagami
  • ,
  • Jun-ichi Nakayama

289
42
開始ページ
28956
終了ページ
28970
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1074/jbc.M114.573725
出版者・発行元
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC

Histone H3K4 methylation has been linked to transcriptional activation. KDM5A (also known as RBP2 or JARID1A), a member of the KDM5 protein family, is an H3K4 demethylase, previously implicated in the regulation of transcription and differentiation. Here, we show that KDM5A is physically and functionally associated with two histone deacetylase complexes. Immunoaffinity purification of KDM5A confirmed a previously described association with the SIN3B-containing histone deacetylase complex and revealed an association with the nucleosome remodeling and deacetylase (NuRD) complex. Sucrose density gradient and sequential immunoprecipitation analyses further confirmed the stable association of KDM5A with these two histone deacetylase complexes. KDM5A depletion led to changes in the expression of hundreds of genes, two-thirds of which were also controlled by CHD4, the NuRD catalytic subunit. Gene ontology analysis confirmed that the genes commonly regulated by both KDM5A and CHD4 were categorized as developmentally regulated genes. ChIP analyses suggested that CHD4 modulates H3K4 methylation levels at the promoter and coding regions of target genes. We further demonstrated that the Caenorhabditis elegans homologues of KDM5 and CHD4 function in the same pathway during vulva development. These results suggest that KDM5A and the NuRD complex cooperatively function to control developmentally regulated genes.

Web of Science ® 被引用回数 : 51

リンク情報
DOI
https://doi.org/10.1074/jbc.M114.573725
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/25190814
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000344141000017&DestApp=WOS_CPL
ID情報
  • DOI : 10.1074/jbc.M114.573725
  • ISSN : 0021-9258
  • eISSN : 1083-351X
  • PubMed ID : 25190814
  • Web of Science ID : WOS:000344141000017

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