論文

査読有り
2016年9月

Identification of Mitosis-Specific Phosphorylation in Mitotic Chromosome-Associated Proteins

JOURNAL OF PROTEOME RESEARCH
  • Shinya Ohta
  • ,
  • Michiko Kimura
  • ,
  • Shunsuke Takagi
  • ,
  • Iyo Toramoto
  • ,
  • Yasushi Ishihama

15
9
開始ページ
3331
終了ページ
3341
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1021/acs.jproteome.6b00512
出版者・発行元
AMER CHEMICAL SOC

During mitosis, phosphorylation of chromosome-associated proteins is a key regulatory mechanism. Mass spectrometry has been successfully applied to determine the complete protein composition of mitotic chromosomes, but not to identify post-translational modifications. Here, we quantitatively compared the phosphoproteome of isolated mitotic chromosomes with that of chromosomes in nonsynchronized cells. We identified 4274 total phosphorylation sites and 350 mitosis-specific phosphorylation sites in mitotic chromosome-associated proteins. Significant mitosis-specific phosphorylation in centromere/kinetochore proteins was detected, although the chromosomal association of these proteins did not change throughout the cell cycle. This mitosis-specific phosphorylation might play a key role in regulation of mitosis. Further analysis revealed strong dependency of phosphorylation dynamics on kinase consensus patterns, thus linking the identified phosphorylation sites to known key mitotic kinases. Remarkably, chromosomal axial proteins such as non-SMC subunits of condensin, TopoII alpha, and Kif4A, together with the chromosomal periphery protein Ki67 involved in the establishment of the mitotic chromosomal structure, demonstrated high phosphorylation during mitosis. These findings suggest a novel mechanism for regulation of chromosome restructuring in mitosis via protein phosphorylation. Our study generated a large quantitative database on protein phosphorylation in mitotic and nonmitotic chromosomes, thus providing insights into the dynamics of chromatin protein phosphorylation at mitosis onset.

Web of Science ® 被引用回数 : 10

リンク情報
DOI
https://doi.org/10.1021/acs.jproteome.6b00512
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27504668
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000382713300036&DestApp=WOS_CPL

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