論文

査読有り
2016年8月

Proteomics Analysis with a Nano Random Forest Approach Reveals Novel Functional Interactions Regulated by SMC Complexes on Mitotic Chromosomes

MOLECULAR & CELLULAR PROTEOMICS
  • Shinya Ohta
  • ,
  • Luis F. Montano-Gutierrez
  • ,
  • Flavia de Lima Alves
  • ,
  • Hiromi Ogawa
  • ,
  • Iyo Toramoto
  • ,
  • Nobuko Sato
  • ,
  • Ciaran G. Morrison
  • ,
  • Shunichi Takeda
  • ,
  • Damien F. Hudson
  • ,
  • Juri Rappsilber
  • ,
  • William C. Earnshaw

15
8
開始ページ
2802
終了ページ
2818
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1074/mcp.M116.057885
出版者・発行元
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC

Packaging of DNA into condensed chromosomes during mitosis is essential for the faithful segregation of the genome into daughter nuclei. Although the structure and composition of mitotic chromosomes have been studied for over 30 years, these aspects are yet to be fully elucidated. Here, we used stable isotope labeling with amino acids in cell culture to compare the proteomes of mitotic chromosomes isolated from cell lines harboring conditional knockouts of members of the condensin (SMC2, CAP-H, CAP-D3), cohesin (Scc1/Rad21), and SMC5/6 (SMC5) complexes. Our analysis revealed that these complexes associate with chromosomes independently of each other, with the SMC5/6 complex showing no significant dependence on any other chromosomal proteins during mitosis. To identify subtle relationships between chromosomal proteins, we employed a nano Random Forest (nanoRF) approach to detect protein complexes and the relationships between them. Our nanoRF results suggested that as few as 113 of 5058 detected chromosomal proteins are functionally linked to chromosome structure and segregation. Furthermore, nanoRF data revealed 23 proteins that were not previously suspected to have functional interactions with complexes playing important roles in mitosis. Subsequent small-interfering-RNA-based validation and localization tracking by green fluorescent protein-tagging highlighted novel candidates that might play significant roles in mitotic progression.

Web of Science ® 被引用回数 : 12

リンク情報
DOI
https://doi.org/10.1074/mcp.M116.057885
J-GLOBAL
https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201702209052607966
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27231315
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000380809100020&DestApp=WOS_CPL

エクスポート
BibTeX RIS