論文

査読有り
2015年4月

CENP-32 is required to maintain centrosomal dominance in bipolar spindle assembly

MOLECULAR BIOLOGY OF THE CELL
  • Shinya Ohta
  • ,
  • Laura Wood
  • ,
  • Iyo Toramoto
  • ,
  • Ken-Ichi Yagyu
  • ,
  • Tatsuo Fukagawa
  • ,
  • William C. Earnshaw

26
7
開始ページ
1225
終了ページ
1237
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1091/mbc.E14-09-1366
出版者・発行元
AMER SOC CELL BIOLOGY

Centrosomes nucleate spindle formation, direct spindle pole positioning, and are important for proper chromosome segregation during mitosis in most animal cells. We previously reported that centromere protein 32 (CENP-32) is required for centrosome association with spindle poles during metaphase. In this study, we show that CENP-32 depletion seems to release centrosomes from bipolar spindles whose assembly they had previously initiated. Remarkably, the resulting anastral spindles function normally, aligning the chromosomes to a metaphase plate and entering anaphase without detectable interference from the free centrosomes, which appear to behave as free asters in these cells. The free asters, which contain reduced but significant levels of CDK5RAP2, show weak interactions with spindle microtubules but do not seem to make productive attachments to kinetochores. Thus CENP-32 appears to be required for centrosomes to integrate into a fully functional spindle that not only nucleates astral microtubules, but also is able to nucleate and bind to kinetochore and central spindle microtubules. Additional data suggest that NuMA tethers microtubules at the anastral spindle poles and that augmin is required for centrosome detachment after CENP-32 depletion, possibly due to an imbalance of forces within the spindle.

Web of Science ® 被引用回数 : 3

リンク情報
DOI
https://doi.org/10.1091/mbc.E14-09-1366
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/25657325
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000351946100003&DestApp=WOS_CPL

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