2010年9月
The Protein Composition of Mitotic Chromosomes Determined Using Multiclassifier Combinatorial Proteomics
CELL
- 巻
- 142
- 号
- 5
- 開始ページ
- 810
- 終了ページ
- 821
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.cell.2010.07.047
- 出版者・発行元
- CELL PRESS
Despite many decades of study, mitotic chromosome structure and composition remain poorly characterized. Here, we have integrated quantitative proteomics with bioinformatic analysis to generate a series of independent classifiers that describe the similar to 4,000 proteins identified in isolated mitotic chromosomes. Integrating these classifiers by machine learning uncovers functional relationships between protein complexes in the context of intact chromosomes and reveals which of the similar to 560 uncharacterized proteins identified here merits further study. Indeed, of 34 GFP-tagged predicted chromosomal proteins, 30 were chromosomal, including 13 with centromere-association. Of 16 GFP-tagged predicted nonchromosomal proteins, 14 were confirmed to be nonchromosomal. An unbiased analysis of the whole chromosome proteome from genetic knockouts of kinetochore protein Ska3/Rama1 revealed that the APC/C and RanBP2/RanGAP1 complexes depend on the Ska complex for stable association with chromosomes. Our integrated analysis predicts that up to 97 new centromere-associated proteins remain to be discovered in our data set.
- リンク情報
- ID情報
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- DOI : 10.1016/j.cell.2010.07.047
- ISSN : 0092-8674
- PubMed ID : 20813266
- Web of Science ID : WOS:000281523200023