論文

査読有り 責任著者
2017年6月

Clustered Xenopus keratin genes: A genomic, transcriptomic, and proteomic analysis

DEVELOPMENTAL BIOLOGY
  • Ken-ichi T. Suzuki
  • ,
  • Miyuki Suzuki
  • ,
  • Mitsuki Shigeta
  • ,
  • Joshua D. Fortriede
  • ,
  • Shuji Takahashi
  • ,
  • Shuuji Mawaribuchi
  • ,
  • Takashi Yamamoto
  • ,
  • Masanori Taira
  • ,
  • Akimasa Fukui

426
2
開始ページ
384
終了ページ
392
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.ydbio.2016.10.018
出版者・発行元
ACADEMIC PRESS INC ELSEVIER SCIENCE

Keratin genes belong to the intermediate filament superfamily and their expression is altered following morphological and physiological changes in vertebrate epithelial cells. Keratin genes are divided into two groups, type I and II, and are clustered on vertebrate genomes, including those of Xenopus species. Various keratin genes have been identified and characterized by their unique expression patterns throughout ontogeny in Xenopus laevis; however, compilation of previously reported and newly identified keratin genes in two Xenopus species is required for our further understanding of keratin gene evolution, not only in amphibians but also in all terrestrial vertebrates. In this study, 120 putative type I and II keratin genes in total were identified based on the genome data from two Xenopus species. We revealed that most of these genes are highly clustered on two homeologous chromosomes, XLA9_10 and XLA2 in X. laevis, and XTR10 and XTR2 in X. tropicalis, which are orthologous to those of human, showing conserved synteny among tetrapods. RNA-Seq data from various embryonic stages and adult tissues highlighted the unique expression profiles of orthologous and homeologous keratin genes in developmental stage- and tissue-specific manners. Moreover, we identified dozens of epidermal keratin proteins from the whole embryo, larval skin, tail, and adult skin using shotgun proteomics. In light of our results, we discuss the radiation, diversification, and unique expression of the clustered keratin genes, which are closely related to epidermal development and terrestrial adaptation during amphibian evolution, including Xenopus speciation.

リンク情報
DOI
https://doi.org/10.1016/j.ydbio.2016.10.018
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000404064800022&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.ydbio.2016.10.018
  • ISSN : 0012-1606
  • eISSN : 1095-564X
  • Web of Science ID : WOS:000404064800022

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