論文

査読有り
2002年1月

A simple selection system for construction of recombinant gD-negative pseudorabies virus as a vaccine vector

VACCINE
  • AL Shiau
  • ,
  • CW Liu
  • ,
  • SY Wang
  • ,
  • CY Tsai
  • ,
  • CL Wu

20
7-8
開始ページ
1186
終了ページ
1195
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/S0264-410X(01)00426-1
出版者・発行元
ELSEVIER SCI LTD

We describe a simple, efficient two-step method for construction of glycoprotein D (gD)-negative pseudorabies virus (PrV) carrying transgenes inserted in place of the gD gene. The first step was the use of the thymidine kinase (TK) gene of herpes simplex virus (HSV) for insertional inactivation of the gD gene in a PrV mutant deficient in both TK and glycoprotein E (gE). The gD-negative, HSV-TK-positive mutant could be selected in HAT medium. The second step was substitution of HSV-TK with other genes of interest. The resultant gD/gE/TK-negative mutant was easily isolated by acyclovir selection. The expression of the transgene was detectable in vivo and the antibody responses against both inserted antigens and PrV were induced. The protective efficacy of the gD/gE/TK-negative PrV against lethal PrV challenge was also demonstrated. This PrV mutant carrying immunogenic proteins from unrelated porcine pathogens may be tested as a multivalent vaccine candidate for swine. (C) 2002 Elsevier Science Ltd. All rights reserved.

Web of Science ® 被引用回数 : 9

リンク情報
DOI
https://doi.org/10.1016/S0264-410X(01)00426-1
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000173858200027&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/S0264-410X(01)00426-1
  • ISSN : 0264-410X
  • Web of Science ID : WOS:000173858200027

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