論文

査読有り
2005年12月

Aqueous dispersions of magnetite nanoparticles with NH3+ surfaces for magnetic manipulations of biomolecules and MRI contrast agents

BIOMATERIALS
  • DB Shieh
  • ,
  • FY Cheng
  • ,
  • CH Su
  • ,
  • CS Yeh
  • ,
  • MT Wu
  • ,
  • YN Wu
  • ,
  • CY Tsai
  • ,
  • CL Wu
  • ,
  • DH Chen
  • ,
  • CH Chou

26
34
開始ページ
7183
終了ページ
7191
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.biomaterials.2005.05.020
出版者・発行元
ELSEVIER SCI LTD

In the current study, amine surface modified iron-oxide nanoparticles of 6 rim diameter without polymer coating were fabricated in an aqueous solution by organic acid modification as an adherent following chemical coprecipitation. Structure and the superparamagnetic property of magnetite nanoparticles were characterized by selected area electron diffraction (SAED) and superconducting quantum interference measurement device (SQUID). X-ray photoelectron spectrometer (XPS) and zeta potential measurements revealed cationic surface mostly decorated with terminal -NH3+. This feature enables them to function as a magnetic carrier for nucleotides via electrostatic interaction. in addition, Fe3O4/trypsin conjugates with well-preserved functional activity was demonstrated. The nanoparticles displayed excellent in vitro biocompatibility. The NMR and the in vitro MRI measurements showed significantly reduced water proton relaxation times of both T-1 and T-2. Significantly reduced T-2 and T-(2)*-weighted signal intensity were observed in a 1.5 T clinical MR imager. In vivo imaging contrast effect showed a fast and prolonged inverse contrast effect in the liver that lasted for more than I week. In addition, it was found that the spherical Fe3O4 assembled as rod-like configuration through an aging process in aqueous solution at room temperature. Interestingly, TEM observation of the liver tissue revealed the rod-like shape but not the spherical-type nanoparticles being taken up by the Kupffer cells 120 h after tail vein infusion. Combining these results, we have demonstrated the potential applications of the newly synthesized magnetite nanoparticles in a broad spectrum of biomedical applications. (c) 2005 Elsevier Ltd. All rights reserved.

Web of Science ® 被引用回数 : 104

リンク情報
DOI
https://doi.org/10.1016/j.biomaterials.2005.05.020
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000231348000040&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.biomaterials.2005.05.020
  • ISSN : 0142-9612
  • Web of Science ID : WOS:000231348000040

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