論文

査読有り 国際誌
2020年1月9日

Self-reactive and polyreactive B cells are generated and selected in the germinal center during γ-herpesvirus infection.

International immunology
  • Shuhei Sakakibara
  • ,
  • Teruhito Yasui
  • ,
  • Hideyuki Jinzai
  • ,
  • Kristy O'donnell
  • ,
  • Chao-Yuan Tsai
  • ,
  • Takeharu Minamitani
  • ,
  • Kazuya Takeda
  • ,
  • Gabrielle T Belz
  • ,
  • David M Tarlinton
  • ,
  • Hitoshi Kikutani

32
1
開始ページ
27
終了ページ
38
記述言語
英語
掲載種別
DOI
10.1093/intimm/dxz057

Immune responses against certain viruses are accompanied by auto-antibody production although the origin of these infection-associated auto-antibodies is unclear. Here, we report that murine γ-herpesvirus 68 (MHV68)-induced auto-antibodies are derived from polyreactive B cells in the germinal center (GC) through the activity of short-lived plasmablasts. The analysis of recombinant antibodies from MHV68-infected mice revealed that about 40% of IgG+ GC B cells were self-reactive, with about half of them being polyreactive. On the other hand, virion-reactive clones accounted for only a minor proportion of IgG+ GC B cells, half of which also reacted with self-antigens. The self-reactivity of most polyreactive clones was dependent on somatic hypermutation (SHM), but this was dispensable for the reactivity of virus mono-specific clones. Furthermore, both virus-mono-specific and polyreactive clones were selected to differentiate to B220lo CD138+ plasma cells (PCs). However, the representation of GC-derived polyreactive clones was reduced and that of virus-mono-specific clones was markedly increased in terminally differentiated PCs as compared to transient plasmablasts. Collectively, our findings demonstrate that, during acute MHV68 infection, self-reactive B cells are generated through SHM and selected for further differentiation to short-lived plasmablasts but not terminally differentiated PCs.

リンク情報
DOI
https://doi.org/10.1093/intimm/dxz057
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31504561
ID情報
  • DOI : 10.1093/intimm/dxz057
  • PubMed ID : 31504561

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