論文

査読有り 国際誌
2018年3月13日

Establishment of Immunodeficient Retinal Degeneration Model Mice and Functional Maturation of Human ESC-Derived Retinal Sheets after Transplantation.

Stem cell reports
  • Satoshi Iraha
  • ,
  • Hung-Ya Tu
  • ,
  • Suguru Yamasaki
  • ,
  • Takahiro Kagawa
  • ,
  • Motohito Goto
  • ,
  • Riichi Takahashi
  • ,
  • Takehito Watanabe
  • ,
  • Sunao Sugita
  • ,
  • Shigenobu Yonemura
  • ,
  • Genshiro A Sunagawa
  • ,
  • Take Matsuyama
  • ,
  • Momo Fujii
  • ,
  • Atsushi Kuwahara
  • ,
  • Akiyoshi Kishino
  • ,
  • Naoshi Koide
  • ,
  • Mototsugu Eiraku
  • ,
  • Hidenobu Tanihara
  • ,
  • Masayo Takahashi
  • ,
  • Michiko Mandai

10
3
開始ページ
1059
終了ページ
1074
記述言語
英語
掲載種別
DOI
10.1016/j.stemcr.2018.01.032

Increasing demand for clinical retinal degeneration therapies featuring human ESC/iPSC-derived retinal tissue and cells warrants proof-of-concept studies. Here, we established two mouse models of end-stage retinal degeneration with immunodeficiency, NOG-rd1-2J and NOG-rd10, and characterized disease progress and immunodeficient status. We also transplanted human ESC-derived retinal sheets into NOG-rd1-2J and confirmed their long-term survival and maturation of the structured graft photoreceptor layer, without rejection or tumorigenesis. We recorded light responses from the host ganglion cells using a multi-electrode array system; this result was consistent with whole-mount immunostaining suggestive of host-graft synapse formation at the responding sites. This study demonstrates an application of our mouse models and provides a proof of concept for the clinical use of human ESC-derived retinal sheets.

リンク情報
DOI
https://doi.org/10.1016/j.stemcr.2018.01.032
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/29503091
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918611