論文

査読有り
2013年3月

Phase geometries of two-dimensional excitable waves govern self-organized morphodynamics of amoeboid cells

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
  • Daisuke Taniguchi
  • ,
  • Shuji Ishihara
  • ,
  • Takehiko Oonuki
  • ,
  • Mai Honda-Kitahara
  • ,
  • Kunihiko Kaneko
  • ,
  • Satoshi Sawai

110
13
開始ページ
5016
終了ページ
5021
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1073/pnas.1218025110
出版者・発行元
NATL ACAD SCIENCES

In both randomly moving Dictyostelium and mammalian cells, phosphatidylinositol (3,4,5)-trisphosphate and F-actin are known to propagate as waves at the membrane and act to push out the protruding edge. To date, however, the relationship between the wave geometry and the patterns of amoeboid shape change remains elusive. Here, by using phase map analysis, we show that morphology dynamics of randomly moving Dictyostelium discoideum cells can be characterized by the number, topology, and position of spatial phase singularities, i.e., points that represent organizing centers of rotating waves. A single isolated singularity near the cellular edge induced a rotational protrusion, whereas a pair of singularities supported a symmetric extension. These singularities appeared by strong phase resetting due to de novo nucleation at the back of preexisting waves. Analysis of a theoretical model indicated excitability of the system that is governed by positive feedback from phosphatidylinositol (3,4,5)-trisphosphate to PI3-kinase activation, and we showed experimentally that this requires F-actin. Furthermore, by incorporating membrane deformation into the model, we demonstrated that geometries of competing waves explain most of the observed semiperiodic changes in amoeboid morphology.

リンク情報
DOI
https://doi.org/10.1073/pnas.1218025110
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/23479620
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000318031900041&DestApp=WOS_CPL
ID情報
  • DOI : 10.1073/pnas.1218025110
  • ISSN : 0027-8424
  • PubMed ID : 23479620
  • Web of Science ID : WOS:000318031900041

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