論文

査読有り
2016年12月

Invariant natural killer T cells play dual roles in the development of experimental autoimmune uveoretinitis

EXPERIMENTAL EYE RESEARCH
  • Masashi Satoh
  • Ken-ichi Namba
  • Nobuyoshi Kitaichi
  • Noriko Endo
  • Hirokuni Kitamei
  • Daiju Iwata
  • Shigeaki Ohno
  • Susumu Ishida
  • Kazunori Onoe
  • Hiroshi Watarai
  • Masaru Taniguchi
  • Tatsuro Ishibashi
  • Joan Stein-Streilein
  • Koh-Hei Sonoda
  • Luc Van Kaer
  • Kazuya Iwabuchi
  • 全て表示

153
開始ページ
79
終了ページ
89
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.exer.2016.10.003
出版者・発行元
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD

Experimental autoimmune uveoretinitis (EAU) represents an experimental model for human endogenous uveitis, which is caused by Th1/Th17 cell-mediated inflammation. Natural killer T (NKT) cells recognize lipid antigens and produce large amounts of cytokines upon activation. To examine the role of NKT cells in the development of uveitis, EAU was elicited by immunization with a peptide from the human interphotoreceptor retinoid-binding protein (hIRBP(1-20)) in complete Freund's adjuvant and histopathology scores were evaluated in C57BL/6 (WT) and NKT cell-deficient mice. NKT cell-deficient mice developed more severe EAU pathology than WT mice. When WT mice were treated with ligands of the invariant subset of NKT cells (alpha-GalCer or RCAI-56), EAU was ameliorated in mice treated with RCAI-56 but not alpha-GalCer. IRBP-specific Thl/Th17 cytokines were reduced in RCAI-56-treated compared with vehicle-treated mice. Although the numbers of IRBP-specific T cells detected by hIRBP(3-13)/I-A(b) tetramers in the spleen and the draining lymph node were the same for vehicle and RCAI-56 treatment groups, ROR gamma t expression by tetramer-positive cells in RCAI-56-treated mice was lower than in control mice. Moreover, the eyes of RCAI-56-treated mice contained fewer IRBP-specific T cells compared with control mice. These results suggest that invariant NKT (iNKT) cells suppress the induction of Th17 cells and infiltration of IRBP-specific T cells into the eyes, thereby reducing ocular inflammation. However, in sharp contrast to the ameliorating effects of iNKT cell activation during the initiation phase of EAU, iNKT cell activation during the effector phase exacerbated disease pathology. Thus, we conclude that iNKT cells exhibit dual roles in the development of EAU. (C) 2016 Elsevier Ltd. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.exer.2016.10.003
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27720708
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000389287700010&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.exer.2016.10.003
  • ISSN : 0014-4835
  • eISSN : 1096-0007
  • PubMed ID : 27720708
  • Web of Science ID : WOS:000389287700010

エクスポート
BibTeX RIS