2019年4月
Collection of biochemical samples with brain-wide electrophysiological recordings from a freely moving rodent
JOURNAL OF PHARMACOLOGICAL SCIENCES
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- 巻
- 139
- 号
- 4
- 開始ページ
- 346
- 終了ページ
- 351
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.jphs.2019.02.006
- 出版者・発行元
- JAPANESE PHARMACOLOGICAL SOC
Bridging accumulating insights from microscopic and macroscopic studies in neuroscience research requires monitoring of neuronal population dynamics and quantifying specific molecules or genes from the brain of identical animals. To this end, by minimizing the size and weight of an electrode array, we developed a method that records local field potential signals of multiple brain regions from one side of the hemisphere in a freely moving rodent. At the same time, extracellular cerebrospinal fluid for biochemical assays or a small part of brain tissue samples for gene expression assays are collected from the other side of the hemisphere. This method allows ongoing stable recordings and sample collections for at least two months. The methodological concept is applicable to a wide range of biological reactions at various spatiotemporal scales, allowing us to integrate an idea of physiolomics into existing omics analyses, leading to a new combination of multi-omics approaches. (c) 2019 The Authors. Production and hosting by Elsevier B. V. on behalf of Japanese Pharmacological Society. This is an open access article under the CC BY-NC-ND license (http://creativecommons. org/ licenses/by-nc-nd/4.0/).
- リンク情報
- ID情報
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- DOI : 10.1016/j.jphs.2019.02.006
- ISSN : 1347-8613
- eISSN : 1347-8648
- PubMed ID : 30871875
- Web of Science ID : WOS:000467698800012