2016年11月25日
Directed evolution of the autoinducer selectivity of Vibrio fischeri LuxR.
The Journal of general and applied microbiology
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- 巻
- 62
- 号
- 5
- 開始ページ
- 240
- 終了ページ
- 247
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.2323/jgam.2016.04.005
- 出版者・発行元
- MICROBIOL RES FOUNDATION
LuxR family transcriptional regulators are the core components of quorum sensing in Gram-negative bacteria and exert their effects through binding to the signaling molecules acyl-homoserine lactones (acyl-HSLs). The function of the LuxR homologs is remarkably plastic, and naturally occurring acyl-HSLs are structurally diverse. To investigate the molecular basis of the functional plasticity of Vibrio fischeri LuxR, we directed the evolution of LuxR toward three different specificities in the laboratory. We found an orthogonal pair of LuxR mutants specific either to 3-oxo-hexanoyl homoserine lactone or to 3-oxo-octanoyl homoserine lactone. Interestingly, the majority of the specificity changes did not arise from modulating the recognition event but rather from changing the efficiency of the transition from the inactive form to the active form upon signal binding. This finding explains how quorum sensing systems can rapidly diverge in nature and in the laboratory and how signal orthogonality and mutual inhibition frequently occur among closely related diverging systems.
- リンク情報
- ID情報
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- DOI : 10.2323/jgam.2016.04.005
- ISSN : 0022-1260
- eISSN : 1349-8037
- PubMed ID : 27725402
- Web of Science ID : WOS:000389424800004