2019年3月
Direct Delivery of Cas9-sgRNA Ribonucleoproteins into Cells Using a Nanoneedle Array
APPLIED SCIENCES-BASEL
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- 巻
- 9
- 号
- 5
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.3390/app9050965
- 出版者・発行元
- MDPI
The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system is a powerful and widely used tool for genome editing. Recently, it was reported that direct delivery of Cas9-sgRNA ribonucleoproteins (RNPs) reduced off-target effects. Therefore, non-invasive, high-throughput methods are needed for direct delivery of RNPs into cells. Here, we report a novel method for direct delivery of RNPs into cells using a nanostructure with a high-aspect-ratio and uniform nanoneedles. This nanostructure is composed of tens of thousands of nanoneedles laid across a 2D array. Through insertion of the nanoneedle array previously adsorbed with Cas9-sgRNA, it was possible to deliver RNPs directly into mammalian cells for genome editing.
- リンク情報
- ID情報
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- DOI : 10.3390/app9050965
- ISSN : 2076-3417
- Web of Science ID : WOS:000462504400152