論文

査読有り
2010年10月

Identification of a Novel Marker for Dendritic Cell Maturation, Mouse Transmembrane Protein 123

JOURNAL OF BIOLOGICAL CHEMISTRY
  • Tomonori Takekoshi
  • ,
  • Yayoi Tada
  • ,
  • Takahiro Watanabe
  • ,
  • Makoto Sugaya
  • ,
  • Toshihiko Hoashi
  • ,
  • Mayumi Komine
  • ,
  • Tomohiko Kawashima
  • ,
  • Teruo Shimizu
  • ,
  • Carren Sy Hau
  • ,
  • Akihiko Asahina
  • ,
  • Takehiko Yokomizo
  • ,
  • Shinichi Sato
  • ,
  • Kunihiko Tamaki

285
41
開始ページ
31876
終了ページ
31884
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1074/jbc.M110.118877
出版者・発行元
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC

Dendritic cells (DCs) are a group of professional antigen-presenting cells, and many genes are known to be associated with their maturation. We compared the transcriptional profiles of immature and mature mouse Langerhans cells using the suppressive, subtractive hybridization method and identified a novel gene of unknown function, termed herein transmembrane protein 123 (Tmem123), of which mRNA expression was enhanced in mature but not in immature Langerhans cells. Its expression was also enhanced in other mature DCs such as bone marrow-derived DCs (BMDCs) and splenic DCs. Interestingly, CD40 expression was up-regulated on mature BMDCs cultured with colchicine concurrently with the enhanced expression of Tmem123 compared with that of fresh BMDCs. Furthermore, the expression of CD40 was enhanced on Tmem123-transfected DC2.4 cells, a mouse BMDC-derived cell line, compared with that on mock-transfected DC2.4 cells. This enhancement of CD40 expression did not occur after deletion of lysosome/endosome targeting YXX phi motifs (where X is any amino acid and phi is a bulky hydrophobic amino acid) in the Tmem123 cytoplasmic tail. By stimulation with anti-CD40 monoclonal antibody, these transfectants secreted an increased amount of IL-12/23 p40 compared with mock-transfected DC2.4 cells. Thus, our study demonstrates that Tmem123 may be used as a new maturation marker in DCs and that this molecule may be closely associated with the cell surface expression of CD40.

Web of Science ® 被引用回数 : 4

リンク情報
DOI
https://doi.org/10.1074/jbc.M110.118877
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/20685650
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000282764600079&DestApp=WOS_CPL

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