論文

査読有り
2003年5月

Human keratinocytes express fractalkine/Cx3CL1

JOURNAL OF DERMATOLOGICAL SCIENCE
  • M Sugaya
  • ,
  • K Nakamura
  • ,
  • H Mitsui
  • ,
  • T Takekoshi
  • ,
  • H Saeki
  • ,
  • K Tamaki

31
3
開始ページ
179
終了ページ
187
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/S0923-1811(03)00031-8
出版者・発行元
ELSEVIER SCI IRELAND LTD

Background: fractalkine/CX3CL1 is a unique chemokine that has properties of both chemoattractants and adhesion molecules. The major source of this chemokine in the skin is still controversial. Objective: studies were undertaken to determine the expression of fractalkine in human skin. Methods: RT-PCR, Western blotting, and immunostaining were performed with normal human epidermal keratinocytes (NHEK) and HaCaT cells, human keratinocyte cell line, for the presence of fractalkine. Biopsy specimens of normal and diseased skin were also investigated. Results: we identified that NHEK and HaCaT cells expressed fractalkine mRNA and protein. The combination of tumor necrosis factor (TNF)-alpha and interferon (IFN)-gamma upregulated their expression by NHEK. Immunohistochemistry demonstrated fractalkine expression in keratinocytes in lichen planus and psoriasis vulgaris. RT-PCR also showed that lesional skin of psoriatic patients expressed higher levels of fractalkine mRNA than non-lesional skin from the same patients. Conclusion: these results suggests that keratinocytes strongly express fractalkine in lichen planus and psoriasis vulgaris and that the fractalkine-CXC3CR1 system in the diseased skin can be a target for the treatment. (C) 2003 Japanese Society of Investigative Dermatology. Published by Elsevier Science Ireland Ltd. All rights reserved. All rights reserved.

Web of Science ® 被引用回数 : 24

リンク情報
DOI
https://doi.org/10.1016/S0923-1811(03)00031-8
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/12727021
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000183172200002&DestApp=WOS_CPL

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