1999年11月
Involvement of thioredoxin peroxidase type II (Ahp1p) of Saccharomyces cerevisiae in Mn2+ homeostasis
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
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- 巻
- 63
- 号
- 11
- 開始ページ
- 1871
- 終了ページ
- 1881
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1271/bbb.63.1871
- 出版者・発行元
- TAYLOR & FRANCIS LTD
To identify new proteins involved in Mn2+ homeostasis, we isolated Mn2+-resistant mutants of Saccharomyces cerevisiae starting from a calcineurin-deficient, Mn2+ hypersensitive strain (Delta cmp1 Delta cmp2). The mutations were found to lie in the PMR1 gene, known to encode a "P-type" Ca2+-ATPase that transports Ca2+ and Mn2+ from the cytosol to the Golgi apparatus. A second gene, AHP1, was cloned as a suppressor of the Mn2+ tolerance of a Delta cmp1 Delta cmp2 pmr1 mutant. Ahp1p was recently described as a thioredoxin peroxidase type II, an antioxidant protein with alkyl hydroperoxide defense properties in yeast. AHP1 disruption in strain W303 decreased tolerance to Mn2+ and H2O2. We found that a GFP-Ahp1p fusion construct was in the cytosol when cells were grown in glucose, and in the mitochondria when cells were grown in oleate. Based on Mn2+ transport data, we concluded that Ahp1p is involved in cellular Mn2+ homeostasis in trafficking of Mn2+ from cytosol to mitochondria and from cytosol for export across the plasma membrane.
- リンク情報
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- DOI
- https://doi.org/10.1271/bbb.63.1871
- CiNii Articles
- http://ci.nii.ac.jp/naid/110002679383
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/10635552
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000084016700004&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1271/bbb.63.1871
- ISSN : 0916-8451
- eISSN : 1347-6947
- CiNii Articles ID : 110002679383
- PubMed ID : 10635552
- Web of Science ID : WOS:000084016700004