Misc.

Sep, 1995

PROTEIN PHOSPHATASE 2B OF SACCHAROMYCES-CEREVISIAE IS REQUIRED FOR TOLERANCE TO MANGANESE, IN BLOCKING THE ENTRY OF IONS INTO THE CELLS

EUROPEAN JOURNAL OF BIOCHEMISTRY
  • IC FARCASANU
  • ,
  • D HIRATA
  • ,
  • E TSUCHIYA
  • ,
  • F NISHIYAMA
  • ,
  • T MIYAKAWA

Volume
232
Number
3
First page
712
Last page
717
Language
English
Publishing type
DOI
10.1111/j.1432-1033.1995.tb20865.x
Publisher
SPRINGER VERLAG

The role of protein phosphatase 2B (PP2B/calcineurin) of Saccharomyces cerevisiae in the tolerance to divalent cations was investigated. PP2B-deficient mutants were found to be sensitive to MnCl2, but not to ZnCl2, CuCl2, NiCl2 and CoCl2. By measuring both manganese uptake and its efflux, it was found that the sensitivity of the mutant cells was due to an increase in manganese uptake and that the wild-type cells were able to prevent manganese entry into the cells, rather than export it in a more efficient manner. In the presence of the immunosuppressant FK506, the behavior of wild-type cells became similar to that of PP2B mutants. Out of various divalent cations tested, externally added magnesium ions were able to block manganese uptake in both wild-type and PP2B mutant strains.

Link information
DOI
https://doi.org/10.1111/j.1432-1033.1995.tb20865.x
CiNii Articles
http://ci.nii.ac.jp/naid/80008581340
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/7588708
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:A1995RV70800004&DestApp=WOS_CPL
ID information
  • DOI : 10.1111/j.1432-1033.1995.tb20865.x
  • ISSN : 0014-2956
  • CiNii Articles ID : 80008581340
  • Pubmed ID : 7588708
  • Web of Science ID : WOS:A1995RV70800004

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