Aug, 2016
Identification of a mutation causing a defective spindle assembly checkpoint in high ethyl caproate-producing sake yeast strain K1801
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
- Volume
- 80
- Number
- 8
- First page
- 1657
- Last page
- 1662
- Language
- Publishing type
- Research paper (scientific journal)
- DOI
- 10.1080/09168451.2016.1184963
- Publisher
- TAYLOR & FRANCIS LTD
In high-quality sake brewing, the cerulenin-resistant sake yeast K1801 with high ethyl caproate-producing ability has been used widely; however, K1801 has a defective spindle assembly checkpoint (SAC). To identify the mutation causing this defect, we first searched for sake yeasts with a SAC-defect like K1801 and found that K13 had such a defect. Then, we searched for a common SNP in only K1801 and K13 by examining 15 checkpoint-related genes in 23 sake yeasts, and found 1 mutation, R48P of Cdc55, the PP2A regulatory B subunit that is important for the SAC. Furthermore, we confirmed that the Cdc55-R48P mutation was responsible for the SAC-defect in K1801 by molecular genetic analyses. Morphological analysis indicated that this mutation caused a high cell morphological variation. But this mutation did not affect the excellent brewing properties of K1801. Thus, this mutation is a target for breeding of a new risk-free K1801 with normal checkpoint integrity.
- Link information
- ID information
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- DOI : 10.1080/09168451.2016.1184963
- ISSN : 0916-8451
- eISSN : 1347-6947