2005年5月
Mutational analysis of the yeast multidrug resistance ABC transporter Pdr5p with altered drug specificity
GENES TO CELLS
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- 巻
- 10
- 号
- 5
- 開始ページ
- 409
- 終了ページ
- 420
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1111/j.1365-2443.2005.00847.x
- 出版者・発行元
- WILEY-BLACKWELL
Multidrug resistance ABC transporter Pdr5p of Saccharomyces cerevisiae is particularly important due to its ability to export a wide range of unrelated substrates. To clarify its function, we generated Pdr5p mutants by random mutagenesis and screened for mutants with altered drug specificity in vivo by using 5 drug compounds. Nine point mutations that caused significant changes in drug specificity distributed throughout the length of Pdr5p, namely, in the extracellular, transmembrane or cytoplasmic regions of the transporter. We then investigated their effects upon drug resistance, using 36 chemically related or distinct substrates. From this study, overall geometry of the Pdr5p was suggested to contribute in acquiring the enormous range of drug specificity. Based on their ability to inhibit the growth of the mutant strains, the 36 tested drugs were classified into: drugs to which the mutants responded differently (Group 1), drugs to which all the mutants showed sensitivity (Group 2), and drugs to which all the mutants exhibited resistance (Group 3). The ability of the compounds to be partitioned to the plasma membrane seemed an important factor for recognition by Pdr5p.
- リンク情報
- ID情報
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- DOI : 10.1111/j.1365-2443.2005.00847.x
- ISSN : 1356-9597
- eISSN : 1365-2443
- Web of Science ID : WOS:000228306800003