2003年2月
An improved method for recovering rabies virus from cloned cDNA.
Journal of virological methods
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- 巻
- 107
- 号
- 2
- 開始ページ
- 229
- 終了ページ
- 36
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/S0166-0934(02)00249-5
- 出版者・発行元
- ELSEVIER SCIENCE BV
A new system for recovery of rabies virus from cDNA plasmid, the transcription of which was driven by cellular RNA polymerase II, was developed. The plasmid contains full-length viral cDNA flanked by hammerhead ribozyme and hepatitis delta ribozyme sequences, arranged downstream of the cytomegalovirus (CMV) promotor. Transfection with the full-length cDNA plasmid together with helper plasmids encoding viral N, P, and L proteins without supply of T7 RNA polymerase produced a recombinant rabies virus in several cell lines. The efficiency of recovery between the conventional T7 promotor system and the new CMV promotor system was compared using these plasmid constructs. The newly established system is applicable to various cell lines and allows rapid and efficient generation of recombinant rabies virus.
- リンク情報
- ID情報
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- DOI : 10.1016/S0166-0934(02)00249-5
- ISSN : 0166-0934
- PubMed ID : 12505638
- Web of Science ID : WOS:000180490500015