論文

査読有り 国際誌
2018年11月

Analysis for genetic loci controlling protoscolex development in the Echinococcus multilocularis infection using congenic mice.

Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
  • Md Atiqul Islam
  • ,
  • Daisuke Torigoe
  • ,
  • Yayoi Kameda
  • ,
  • Takao Irie
  • ,
  • Hirokazu Kouguchi
  • ,
  • Ryo Nakao
  • ,
  • Md Abdul Masum
  • ,
  • Osamu Ichii
  • ,
  • Yasuhiro Kon
  • ,
  • Hassan T Tag-El-Din-Hassan
  • ,
  • Masami Morimatsu
  • ,
  • Kinpei Yagi
  • ,
  • Takashi Agui

65
開始ページ
65
終了ページ
71
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.meegid.2018.07.017

The resistance/susceptibility to Echinococcus multilocularis infection in mice is genetically controlled. However, genetic factors responsible for these differences remain unknown. Our previous study in genetic linkage analysis has revealed that there is a significant quantitative trait locus (QTL) for the establishment of cyst (Emcys1), and a highly significant QTL for the development of protoscolex of E. multilocularis larvae (Empsc1), on mouse chromosomes 6 and 1, respectively. The current study aimed to confirm these QTLs and narrow down the critical genetic region that controls resistance/susceptibility to E. multilocularis infection by establishing congenic and subcongenic lines from C57BL/6 (B6) and DBA/2 (D2) mice. For protoscolex development phenotype, two congenic lines, B6.D2-Empsc1 and D2.B6-Empsc1 were developed, where responsible QTL, Empsc1 was introgressed from D2 into B6 background and vice versa. For cyst establishment phenotype, two congenic lines, B6.D2-Emcys1 and D2.B6-Emcys1 were developed, where responsible QTL, Emcys1 was introgressed from D2 into B6 background and vice versa. Because there was no significant difference in cyst establishment between B6.D2-Emcys1 and D2.B6-Emcys1 mice after challenge with E. multilocularis, it is suggested that the Emcys1 does not solely control the cyst establishment in mouse liver. However, infection experiments with B6.D2-Empsc1 and D2.B6-Empsc1 mice showed a significant difference in protoscolex development in the cyst. It confirms that the Empsc1 controls phenotype of the protoscolex development in the cyst. Subsequently, two subcongenic lines, B6.D2-Empsc1.1 and B6.D2-Empsc1.2 from B6.D2-Emcys1 and one subcongenic line, D2.B6-Empsc1.1 from D2.B6-Empsc1 were developed to narrow down the critical region responsible for protoscolex development. From the results of infection experiments with E. multilocularis in these subcongenic mice, it is concluded that a gene responsible for protoscolex development is located between D1Mit290 (68.1 cM) and D1Mit511 (97.3 cM).

リンク情報
DOI
https://doi.org/10.1016/j.meegid.2018.07.017
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30030204
ID情報
  • DOI : 10.1016/j.meegid.2018.07.017
  • ISSN : 1567-1348
  • PubMed ID : 30030204

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