論文

査読有り
2014年2月

Development of an ELISA Using a Recombinant P46-Like Lipoprotein for Diagnosis of Mycoplasma pulmonis Infection in Rodents

JOURNAL OF VETERINARY MEDICAL SCIENCE
  • Atsushi Asano
  • ,
  • Daisuke Torigoe
  • ,
  • Nobuya Sasaki
  • ,
  • Takashi Agui

76
2
開始ページ
151
終了ページ
157
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1292/jvms.13-0308
出版者・発行元
JAPAN SOC VET SCI

Mycoplasma pulmonis is one of the most prevalent bacterial pathogens that infects laboratory mice and rats. To develop an M. pulmonis-specific antigen for serological diagnosis, we cloned the cDNA of P46-like lipoprotein (P46L), an M. pulmonis putative periplasmic protein. P46L is a homolog of P46, an M. hyopneumoniae antigen. We produced recombinant P46L fused to glutathione S-transferase (GST) in Escherichia coli. Immunoblot analysis revealed that sera from Mycoplasma-infected mice and rats contained anti-P46L antibodies. We developed an ELISA using the recombinant P46L-GST protein as an antigen. Thirteen of the 14 samples from rats naturally infected with M. pulmonis were determined to be positive according to the commercial ELISA (MONILISA Myco) and positive by our ELISA. Furthermore, 18/19 samples from mice experimentally infected with M. pulmonis were positive using our P46L-GST ELISA. In contrast, only 8/19 samples from infected mice were positive by the commercial ELISA. Our results indicate that P46L-GST was an appropriate antigen for developing a serological test to determine M. pulmonis infection in laboratory mice and rats.

Web of Science ® 被引用回数 : 4

リンク情報
DOI
https://doi.org/10.1292/jvms.13-0308
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24065083
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000332719500001&DestApp=WOS_CPL
ID情報
  • DOI : 10.1292/jvms.13-0308
  • ISSN : 0916-7250
  • eISSN : 1347-7439
  • PubMed ID : 24065083
  • Web of Science ID : WOS:000332719500001

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