論文

査読有り
2013年7月

Epitope Mapping of the Nucleocapsid Protein of Sendai Virus and Application of Antigenic Epitopes for the ELISA-Based Diagnosis of Sendai Virus Infection

JOURNAL OF VETERINARY MEDICAL SCIENCE
  • Atsushi Asano
  • ,
  • Daisuke Torigoe
  • ,
  • Nobuya Sasaki
  • ,
  • Takashi Agui

75
7
開始ページ
909
終了ページ
916
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1292/jvms.12-0496
出版者・発行元
JAPAN SOC VET SCI

Sendai virus (SeV) is one of the most prevalent viral pathogens infecting laboratory mice and rats. To date, mature SeV virions have been used as antigens for serological diagnosis. To develop antigens that are more specific and easier to prepare for diagnosis, we examined the antigenic sites in the nucleocapsid protein (NP) of SeV with antisera from experimentally SeV-infected mice and a peptide array membrane containing overlapping 10-mer peptides covering the entire NP. We found antigenic linear sequences in two regions, amino acids 120-160 and 420-500, of the SeV-NP. From these antigenic sequences, we applied two synthesized peptides, IVKTRDMEYERTTEWL and FVTLHGAERLEEETNDE, which correspond to positions 119-134 and 458-474 of the SeV-NP, respectively, as antigens in an enzyme-linked immunosorbent assay (ELISA). Evaluation of the ELISAs using these peptides revealed that they were specific to anti-SeV antisera. Furthermore, the ELISAs using these peptides were able to distinguish between SeV-positive and SeV-negative mouse sera to the same extent as a commercial ELISA kit. These results indicate that these peptides are useful for the serological diagnosis of SeV infection.

Web of Science ® 被引用回数 : 1

リンク情報
DOI
https://doi.org/10.1292/jvms.12-0496
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/23446107
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000330268400009&DestApp=WOS_CPL
ID情報
  • DOI : 10.1292/jvms.12-0496
  • ISSN : 0916-7250
  • eISSN : 1347-7439
  • PubMed ID : 23446107
  • Web of Science ID : WOS:000330268400009

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