2014年9月
Simultaneous visualization of extrinsic and intrinsic axon collaterals in Golgi-like detail for mouse corticothalamic and corticocortical cells: a double viral infection method
FRONTIERS IN NEURAL CIRCUITS
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- 巻
- 8
- 号
- 開始ページ
- 110
- 終了ページ
- 110
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.3389/fncir.2014.00110
- 出版者・発行元
- FRONTIERS RESEARCH FOUNDATION
Here we present a novel tracing technique to stain projection neurons in Golgi-like detail by double viral infection. We used retrograde lentiviral vectors and adeno-associated viral vectors (AAV) to drive "TET-ON/TET-OFF system" in neurons connecting two regions. Using this method, we successfully labeled the corticothalamic (CT) cells of the mouse somatosensory barrel field (S1BF) and motor cortex (M1) in their entirety. We also labeled contra- and ipsilaterally-projecting corticocortical (CC) cells of M1 by targeting contralateral M1 or ipsilateral Si for retrograde infection. The strength of this method is that we can observe the morphology of specific projection neuron subtypes en masse. We found that the group of CT cells extends their dendrites and intrinsic axons extensively below but not within the thalamorecipient layer in both Si BE and M1, suggesting that the primary target of this cell type is not layer 4. We also found that both ipsi- and contralateral targeting CC cells in M1 commonly exhibit widespread collateral extensions to contralateral M1 (layers 1-6), bilateral Si and S2 (layers 1, 5 and 6), perirhinal cortex (layers 1, 2/3, 5, and 6), striatum and claustrum. These findings not only strengthened the previous findings of single cell tracings but also extended them by enabling cross-area comparison of CT cells or comparison of CC cells of two different labeling.
- リンク情報
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- DOI
- https://doi.org/10.3389/fncir.2014.00110
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/25278843
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4166322
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000344000100001&DestApp=WOS_CPL
- ID情報
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- DOI : 10.3389/fncir.2014.00110
- ISSN : 1662-5110
- PubMed ID : 25278843
- PubMed Central 記事ID : PMC4166322
- Web of Science ID : WOS:000344000100001