2012年7月
Genetic dissection of the circuit for hand dexterity in primates
NATURE
- 巻
- 487
- 号
- 7406
- 開始ページ
- 235
- 終了ページ
- U1510
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/nature11206
- 出版者・発行元
- NATURE PUBLISHING GROUP
It is generally accepted that the direct connection from the motor cortex to spinal motor neurons is responsible for dexterous hand movements in primates(1-3). However, the role of the 'phylogenetically older' indirect pathways from the motor cortex to motor neurons, mediated by spinal interneurons, remains elusive. Here we used a novel double-infection technique to interrupt the transmission through the propriospinal neurons (PNs)(4-6), which act as a relay of the indirect pathway in macaque monkeys (Macaca fuscata and Macaca mulatta). The PNs were double infected by injection of a highly efficient retrograde gene-transfer vector into their target area and subsequent injection of adeno-associated viral vector at the location of cell somata. This method enabled reversible expression of green fluorescent protein (GFP)-tagged tetanus neurotoxin, thereby permitting the selective and temporal blockade of the motor cortex-PN-motor neuron pathway. This treatment impaired reach and grasp movements, revealing a critical role for the PN-mediated pathway in the control of hand dexterity. Anti-GFP immunohistochemistry visualized the cell bodies and axonal trajectories of the blocked PNs, which confirmed their anatomical connection to motor neurons. This pathway-selective and reversible technique for blocking neural transmission does not depend on cell-specific promoters or transgenic techniques, and is a new and powerful tool for functional dissection in system-level neuroscience studies.
- リンク情報
-
- DOI
- https://doi.org/10.1038/nature11206
- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201202227483844893
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/22722837
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000306278900040&DestApp=WOS_CPL
- ID情報
-
- DOI : 10.1038/nature11206
- ISSN : 0028-0836
- J-Global ID : 201202227483844893
- PubMed ID : 22722837
- Web of Science ID : WOS:000306278900040