論文

査読有り
2011年2月

ERK5 Regulates Muscle Cell Fusion through Klf Transcription Factors

DEVELOPMENTAL CELL
  • Kazunori Sunadome
  • ,
  • Takuya Yamamoto
  • ,
  • Miki Ebisuya
  • ,
  • Kunio Kondoh
  • ,
  • Atsuko Sehara-Fujisawa
  • ,
  • Eisuke Nishida

20
2
開始ページ
192
終了ページ
205
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.devcel.2010.12.005
出版者・発行元
CELL PRESS

In skeletal muscle differentiation, muscle-specific genes are regulated by two groups of transcription factors, the MyoD and MEF2 families, which work together to drive the differentiation process. Here, we show that ERK5 regulates muscle cell fusion through Klf transcription factors. The inhibition of ERK5 activity suppresses muscle cell fusion with minimal effects on the expression of MyoD, MEF2, and their target genes. Promoter analysis coupled to microarray assay reveals that Klf-binding motifs are highly enriched in the promoter regions of ERK5-dependent upregulated genes. Remarkably, Klf2 and Klf4 expression are also upregulated during differentiation in an ERK5-dependent manner, and knockdown of Klf2 or Klf4 specifically suppresses muscle cell fusion. Moreover, we show that Sp1 transcription factor links ERK5 to Klf2/4, and that nephronectin, a Klf transcriptional target, is involved in muscle cell fusion. Therefore, an ERK5/Sp1/Klf module plays a key role in the fusion process during skeletal muscle differentiation.

リンク情報
DOI
https://doi.org/10.1016/j.devcel.2010.12.005
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000287646800008&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.devcel.2010.12.005
  • ISSN : 1534-5807
  • Web of Science ID : WOS:000287646800008

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