論文

査読有り
2014年8月

Calyculin biogenesis from a pyrophosphate protoxin produced by a sponge symbiont

NATURE CHEMICAL BIOLOGY
  • Toshiyuki Wakimoto
  • Yoko Egami
  • Yu Nakashima
  • Yukihiko Wakimoto
  • Takahiro Mori
  • Takayoshi Awakawa
  • Takuya Ito
  • Hiromichi Kenmoku
  • Yoshinori Asakawa
  • Joern Piel
  • Ikuro Abe
  • 全て表示

10
8
開始ページ
648
終了ページ
U193
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/NCHEMBIO.1573
出版者・発行元
NATURE PUBLISHING GROUP

The Japanese marine sponge Discodermia calyx contains a major cytotoxic compound, calyculin A, which exhibits selective inhibition of protein phosphatases 1 and 2A. It has long been used as a chemical tool to evaluate intracellular signal transduction regulated by reversible protein phosphorylation. We describe the identification of the biosynthetic gene cluster of calyculin A by a metagenome mining approach. Single-cell analysis revealed that the gene cluster originates in the symbiont bacterium 'Candidatus Entotheonella' sp. A phosphotransferase encoded in the gene cluster deactivated calyculin A to produce a newly discovered diphosphate, which was actually the biosynthetic end product. The diphosphate had been previously overlooked because of the enzymatic dephosphorylation that occurred in response to sponge tissue disruption. Our work presents what is to our knowledge the first evidence for the biosynthetic process of calyculin A along with a notable phosphorylation-dephosphorylation mechanism to regulate toxicity, suggesting activated chemical defense in the most primitive of all multicellular animals.

リンク情報
DOI
https://doi.org/10.1038/NCHEMBIO.1573
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000339380100011&DestApp=WOS_CPL
ID情報
  • DOI : 10.1038/NCHEMBIO.1573
  • ISSN : 1552-4450
  • eISSN : 1552-4469
  • Web of Science ID : WOS:000339380100011

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