2018年
Culture of mouse giant central nervous system synapses and application for imaging and electrophysiological analyses
Methods in Molecular Biology
- ,
- ,
- ,
- 巻
- 1727
- 号
- 開始ページ
- 201
- 終了ページ
- 215
- 記述言語
- 英語
- 掲載種別
- 研究論文（学術雑誌）
- DOI
- 10.1007/978-1-4939-7571-6_15
- 出版者・発行元
- Humana Press Inc.
Primary neuronal cell culture preparations are widely used to investigate synaptic functions. This chapter describes a detailed protocol for the preparation of a neuronal cell culture in which giant calyx-type synaptic terminals are formed. This chapter also presents detailed protocols for utilizing the main technical advantages provided by such a preparation, namely, labeling and imaging of synaptic organelles and electrophysiological recordings directly from presynaptic terminals.
- リンク情報
- ID情報
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- DOI : 10.1007/978-1-4939-7571-6_15
- ISSN : 1064-3745
- ORCIDのPut Code : 75615877
- PubMed ID : 29222783
- SCOPUS ID : 85037683629