論文

査読有り
2017年

Directional migration of MDA-MB-231 cells under O2/pH gradients

Advances in Experimental Medicine and Biology
  • Y. Enokida
  • ,
  • Y. Tsuruno
  • ,
  • K. Okubo
  • ,
  • Y. Yamaoka
  • ,
  • E. Takahashi

977
開始ページ
169
終了ページ
174
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/978-3-319-55231-6_23
出版者・発行元
Springer New York LLC

We hypothesized that cancer cells actively migrate toward intratumor microvessels, guided by tissue gradients of metabolic substrates (such as O2) and/or metabolites (such as CO2/H+). To test this hypothesis, we developed an in vitro model in which cellular energy metabolism establishes gradients of O2/nutrient/ metabolite in monolayer cells cultured in a conventional culture dish. When gradients of O2 ranging from 3% to ~0% were produced, MDA-MB-231 cells located at 300, 500 and 1500 μm downstream in the gradient demonstrated significant directional migrations (Rayleigh z test). We also found a similar directionality in cell migration at the same location even when the initial O2 level in the O2 gradient was raised from 3% to 21%. Interestingly, such directionalities were no longer demonstrated when the cell density was lowered from 1.8 × 106 to 0.9 × 106 cells/ml. In the former, the magnitude of the extracellular pH gradient in regions 300 and 500 μm downstream in the gradient was significantly larger. Thus, the direction of cell migrations appeared to depend on the gradient of extracellular pH rather than on O2.

リンク情報
DOI
https://doi.org/10.1007/978-3-319-55231-6_23
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28685442
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85026358412&origin=inward
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85026358412&origin=inward
ID情報
  • DOI : 10.1007/978-3-319-55231-6_23
  • ISSN : 2214-8019
  • ISSN : 0065-2598
  • eISSN : 2214-8019
  • PubMed ID : 28685442
  • SCOPUS ID : 85026358412

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