論文

査読有り
2005年2月

Activated human umbilical cord blood dendritic cells kill tumor cells without damaging normal hematological progenitor cells

CANCER SCIENCE
  • J Shi
  • ,
  • K Ikeda
  • ,
  • N Fujii
  • ,
  • E Kondo
  • ,
  • K Shinagawa
  • ,
  • F Ishimaru
  • ,
  • K Kaneda
  • ,
  • M Tanimoto
  • ,
  • Li, X
  • ,
  • Q Pu

96
2
開始ページ
127
終了ページ
133
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/j.1349-7006.2005.00017.x
出版者・発行元
WILEY-BLACKWELL

Apart from their role as antigen presenting cells, human peripheral blood monocyte and CD34+ cell-derived dendritic cells (DC), have been demonstrated to exert cytotoxicity against some tumor cells, and their tumoricidal activity can be enhanced by some stimili. However, there have been no reports concerning the tumoricidal activity of human cord blood dendritic cells (CBDC). In this article, we report that human cord blood monocyte-derived DC acquire the ability to kill hematological tumor cells, after activation with lipopolysaccharicle (LIPS) or gamma-interferon (IFN-gamma), associated with the enhanced TNF-alpha-related apoptosis-inducing ligand (TRAIL) expression in CBDC cytoplasm. The CD14-positive cells collected from cord blood were induced to CBDC in vitro. After activation with IFN-gamma for 12 h, CBDC exhibited cytotoxicity against HL60 and Jurkat cells, while activation with LPS induced cytotoxicity against Daudi and Jurkat cells. However, both LPS- and IFN-gamma-stimulatecl CBDC showed no cytotoxic activity against normal CD14-negative cord blood mononuclear cells. The formation of umbilical cord hematopoietic progenitor colonies, identified as burst-forming unit-erythroid and colony-forming unit granulocyte-macrophage, was not inhibited by stimulated or unstimulated CBDC. IFN-gamma or LPS stimulation enhanced intracellular but not cellular surface TRAIL, and neither intracellular nor cellular surface tumor necrosing factor-alpha and Fas Ligand as analyzed by flow cytometry. Our results show that activated CBDC can serve as cytotoxic cells against hematological tumor cells without damaging the normal hematopoietic progenitor cells.

Web of Science ® 被引用回数 : 29

リンク情報
DOI
https://doi.org/10.1111/j.1349-7006.2005.00017.x
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000227826400010&DestApp=WOS_CPL
URL
http://www.scopus.com/inward/record.url?eid=2-s2.0-20144362968&partnerID=MN8TOARS
URL
http://orcid.org/0000-0003-0995-9405

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