論文

査読有り
2002年12月

Targeted cloning of cytotoxic T cells specific for minor histocompatibility antigens restricted by HLA class I molecules of interest

TRANSPLANTATION
  • Y Akatsuka
  • ,
  • E Kondo
  • ,
  • H Taji
  • ,
  • Y Morishima
  • ,
  • M Yazaki
  • ,
  • Y Obata
  • ,
  • Y Kodera
  • ,
  • Riddell, SR
  • ,
  • T Takahashi

74
12
開始ページ
1773
終了ページ
1780
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1097/01.TP.0000039166.86548.93
出版者・発行元
LIPPINCOTT WILLIAMS & WILKINS

Background. T cells specific for minor histocompatibility antigens (mHAgs) play a major role in both graft-versus-host disease and the graft-versus-tumor effect after HLA-identical bone marrow transplantation (BMT). However, characterization of individual T-cell responses to mHAgs is difficult and has generally involved extensive screening of T-cell clones isolated from bulk T-cell cultures generated from BMT recipients. In this report, we describe a new approach that permits both direct visualization of CD8(+) T-cell responses to mHAgs and cloning of T cells reacting with mHAgs presented by individual HLA alleles of interest.
Methods and Results. Panels of Epstein-Barr virus. transformed B-cell lines (B-LCL) expressing retrovirally transduced HLA cDNA were used as stimulator cells in an enzyme-linked immunospot (ELISPOT) assay to identify CD8(+) T cells reacting with mHAgs in cultures generated from postBMT recipient peripheral blood. T cells specific for mHAgs presented by selected HLA alleles could then be captured and cloned using an interferon-gamma secretion assay and magnetic bead selection. A majority of T-cell clones thus isolated exhibited cytolytic activity against the same HLA-transfected B-cell lines used for the ELISPOT assay.
Conclusion. The ELISPOT assay was useful for identification of the HLA alleles presenting mHAgs recognized by individual T-cell lines. This approach for isolating mHAgs-specific CD8(+) T-cell clones should assist in characterizing responses restricted by HLA alleles of interest, which are common in a certain ethnic group.

Web of Science ® 被引用回数 : 10

リンク情報
DOI
https://doi.org/10.1097/01.TP.0000039166.86548.93
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000180044000023&DestApp=WOS_CPL
URL
http://www.scopus.com/inward/record.url?eid=2-s2.0-0037184879&partnerID=MN8TOARS
URL
http://orcid.org/0000-0003-0995-9405

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