2002年8月
Efficient generation of antigen-specific cytotoxic T cells using retrovirally transduced CD40-activated B cells
JOURNAL OF IMMUNOLOGY
- ,
- ,
- ,
- ,
- ,
- ,
- ,
- ,
- ,
- 巻
- 169
- 号
- 4
- 開始ページ
- 2164
- 終了ページ
- 2171
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.4049/jimmunol.169.4.2164
- 出版者・発行元
- AMER ASSOC IMMUNOLOGISTS
The development of rapid, efficient, and safe methods for generating Ag-specific T cells is necessary for the clinical application of adoptive immunotherapy. We show that B cells stimulated with CD40 ligand and IL-4 (CD40-B cells) can be efficiently transduced with retroviral vectors encoding a model Ag, CMV tegument protein pp65 gene, and maintain high levels of costimulatory molecules after gene transfer. CTL lines specific for pp65 were readily generated in all four healthy CMV-seropositive donors by stimulating autologous CD8(+) T cells with these transduced CD40-B cells, both of which were derived from 10 ml peripheral blood. ELISPOT assays revealed that the CTL lines used multiple HLA alleles as restricting elements. Thus, CD40-B cells transduced retrovirally with Ag-encoding cDNA can be potent APC and facilitate to generate Ag-specific CTL in vitro.
- リンク情報
-
- DOI
- https://doi.org/10.4049/jimmunol.169.4.2164
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000177365800063&DestApp=WOS_CPL
- URL
- http://www.scopus.com/inward/record.url?eid=2-s2.0-0037103241&partnerID=MN8TOARS
- URL
- http://orcid.org/0000-0003-0995-9405
- ID情報
-
- DOI : 10.4049/jimmunol.169.4.2164
- ISSN : 0022-1767
- ORCIDのPut Code : 47323620
- SCOPUS ID : 0037103241
- Web of Science ID : WOS:000177365800063