論文

査読有り
2002年8月

Efficient generation of antigen-specific cytotoxic T cells using retrovirally transduced CD40-activated B cells

JOURNAL OF IMMUNOLOGY
  • E Kondo
  • ,
  • MS Topp
  • ,
  • HP Kiem
  • ,
  • Y Obata
  • ,
  • Y Morishima
  • ,
  • K Kuzushima
  • ,
  • M Tanimoto
  • ,
  • M Harada
  • ,
  • T Takahashi
  • ,
  • Y Akatsuka

169
4
開始ページ
2164
終了ページ
2171
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.4049/jimmunol.169.4.2164
出版者・発行元
AMER ASSOC IMMUNOLOGISTS

The development of rapid, efficient, and safe methods for generating Ag-specific T cells is necessary for the clinical application of adoptive immunotherapy. We show that B cells stimulated with CD40 ligand and IL-4 (CD40-B cells) can be efficiently transduced with retroviral vectors encoding a model Ag, CMV tegument protein pp65 gene, and maintain high levels of costimulatory molecules after gene transfer. CTL lines specific for pp65 were readily generated in all four healthy CMV-seropositive donors by stimulating autologous CD8(+) T cells with these transduced CD40-B cells, both of which were derived from 10 ml peripheral blood. ELISPOT assays revealed that the CTL lines used multiple HLA alleles as restricting elements. Thus, CD40-B cells transduced retrovirally with Ag-encoding cDNA can be potent APC and facilitate to generate Ag-specific CTL in vitro.

Web of Science ® 被引用回数 : 88

リンク情報
DOI
https://doi.org/10.4049/jimmunol.169.4.2164
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000177365800063&DestApp=WOS_CPL
URL
http://www.scopus.com/inward/record.url?eid=2-s2.0-0037103241&partnerID=MN8TOARS
URL
http://orcid.org/0000-0003-0995-9405

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