論文

査読有り
2003年6月

Identification of a polymorphic gene, BCL2A1, encoding two novel hematopoietic lineage-specific minor histocompatibility antigens

JOURNAL OF EXPERIMENTAL MEDICINE
  • Y Akatsuka
  • ,
  • T Nishida
  • ,
  • E Kondo
  • ,
  • M Miyazaki
  • ,
  • H Taji
  • ,
  • H Iida
  • ,
  • K Tsujimura
  • ,
  • M Yazaki
  • ,
  • T Naoe
  • ,
  • Y Morishima
  • ,
  • Y Kodera
  • ,
  • K Kuzushima
  • ,
  • T Takahashi

197
11
開始ページ
1489
終了ページ
1500
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1084/jem.20021925
出版者・発行元
ROCKEFELLER UNIV PRESS

We report the identification of two novel minor histocompatibility antigens (mHAgs), encoded by two separate single nucleotide polymorphisms on a single gene, BCL2A1, and restricted by human histocompatibility leukocyte antigen (HLA)-A*2402 (the most common HLA-A allele in Japanese) and B*4403, respectively. Two cytotoxic T lymphocyte (CTL) clones specific for these mHAgs were first isolated from two distinct recipients after hematopoietic cell transplantation. Both clones lyse only normal and malignant cells within the hematopoietic lineage. To localize the gene encoding the mHAgs, two-point linkage analysis was performed on the CTL lytic patterns of restricting HLA-transfected B lymphoblastoid cell lines obtained from Centre d'Etude du Polymorphisme Humain. Both CTL clones showed a completely identical lyric pattern for 4 pedigrees and the gene was localized within a 3.6-cM interval of 15q24.3-25.1 region that encodes at least 46 genes. Of those, only BCL2A1 has been reported to be expressed in hematopoietic cells and possess three nonsynonymous nucleotide changes. Mini-gene transfection and epitope reconstitution assays with synthetic peptides identified both HLA-A*2402- and B*4403-restricted mHAg epitopes to be encoded by distinct polymorphisms within BCL2A1.

Web of Science ® 被引用回数 : 112

リンク情報
DOI
https://doi.org/10.1084/jem.20021925
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000183376300009&DestApp=WOS_CPL
URL
http://www.scopus.com/inward/record.url?eid=2-s2.0-12444312572&partnerID=MN8TOARS
URL
http://orcid.org/0000-0003-0995-9405

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