2010年11月
Mechanistic Studies of the Biosynthesis of 2-Thiosugar: Evidence for the Formation of an Enzyme-Bound 2-Ketohexose Intermediate in BexX-Catalyzed Reaction
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
- ,
- 巻
- 132
- 号
- 44
- 開始ページ
- 15544
- 終了ページ
- 15546
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1021/ja108061c
- 出版者・発行元
- AMER CHEMICAL SOC
The first mechanistic insight into 2-thiosugar production in an angucycline-type antibiotic, BE-7585A, is reported. D-Glucose 6-phosphate was identified as the substrate for the putative thiosugar biosynthetic protein, BexX, by trapping the covalently bonded enzyme-substrate intermediate. The site-specific modification at K110 residue was determined by mutagenesis studies and LC-MS/MS analysis. A key intermediate carrying a keto functionality was confirmed to exist in the enzyme-substrate complex. These results suggest that the sulfur insertion mechanism in 2-thiosugar biosynthesis shares similarities with that for thiamin biosynthesis.
- リンク情報
- ID情報
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- DOI : 10.1021/ja108061c
- ISSN : 0002-7863
- PubMed ID : 20961106
- Web of Science ID : WOS:000283955600030